Characterization of the UDP-glucose pyrophosphorylase gene from the marine red alga Gracilaria gracilis

Lluisma A.O. and Ragan M.A. (1998) Characterization of the UDP-glucose pyrophosphorylase gene from the marine red alga Gracilaria gracilis. Journal of Applied Phycology, 10 6: 581-588. doi:10.1023/A:1008050421875


Author Lluisma A.O.
Ragan M.A.
Title Characterization of the UDP-glucose pyrophosphorylase gene from the marine red alga Gracilaria gracilis
Journal name Journal of Applied Phycology   Check publisher's open access policy
ISSN 0921-8971
Publication date 1998-01-01
Year available 1998
Sub-type Article (original research)
DOI 10.1023/A:1008050421875
Open Access Status Not yet assessed
Volume 10
Issue 6
Start page 581
End page 588
Total pages 8
Place of publication DORDRECHT
Publisher KLUWER ACADEMIC PUBL
Language eng
Subject 1104 Complementary and Alternative Medicine
1305 Biotechnology
Abstract UDP-glucose pyrophosphorylase (UGPase) is a key enzyme in carbohydrate metabolism, particularly polysaccharide biosynthesis, in red algae. In this report, we characterize at the genomic and cDNA levels the putative UGPase gene of the agarophytic red alga Gracilaria gracilis. The gene is single-copy, devoid of introns, and produces two kinds of transcripts that differ in size by 332 basepairs. The large and small transcripts appear to utilize distinct polyadenylation signals. The putative protein has 495 amino acids, and is about 50% identical in sequence to its homplogs in plants, animals and fungi. Sequencing of the,genomic clone revealed that another gene, potentially encoding a DNA helicase and containing a 76 bp-intron near its 3' end, occurs 376 bp downstream of the UGPase gene.
Keyword Polysaccharide biosynthesis
Red algae
UDPglucose pyrophosphorylase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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