Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2

Sensen C.W., Klenk H.-P., Singh R.K., Allard G., Chan C.C.-Y., Liu Q.Y., Penny S.L., Young F., Schenk M.E., Gaasterland T., Doolittle W.F., Ragan M.A. and Charlebois R.L. (1996) Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2. Molecular Microbiology, 22 1: 175-191. doi:10.1111/j.1365-2958.1996.tb02666.x


Author Sensen C.W.
Klenk H.-P.
Singh R.K.
Allard G.
Chan C.C.-Y.
Liu Q.Y.
Penny S.L.
Young F.
Schenk M.E.
Gaasterland T.
Doolittle W.F.
Ragan M.A.
Charlebois R.L.
Title Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
Publication date 1996-01-01
Year available 1996
Sub-type Article (original research)
DOI 10.1111/j.1365-2958.1996.tb02666.x
Open Access Status Not yet assessed
Volume 22
Issue 1
Start page 175
End page 191
Total pages 17
Place of publication OXFORD
Publisher BLACKWELL SCIENCE LTD
Language eng
Subject 1312 Molecular Biology
2404 Microbiology
Abstract We have initiated a project to sequence the 3 Mbp genome of the thermoacidophilic archaebacterium Sulfolobus solfataricus P2. Cosmids were selected from a provisional set of minimally overlapping clones, subcloned in pUC18, and sequenced using a hybrid (random plus directed) strategy to give two blocks of contiguous unique sequence, respectively, 100 389 and 56 105bp. These two contigs contain a total of 163 open reading frames (ORFs) in 26-29 putative operons; 56 ORFs could be identified with reasonable certainty. Clusters of ORFs potentially encode proteins of glycogen biosynthesis, oxidative decarboxylation of pyruvate, ATP-dependent transport across membranes, isoprenoid biosynthesis, protein synthesis, and ribosomes. Putative promoters occur upstream of most ORFs. Thirty per cent of the predicted strong and medium-strength promoters can initiate transcription at the start codon or within 10 nucleotides upstream, indicating a process of initial mRNA-ribosome contact unlike that of most eubacterial genes. A novel termination motif is proposed to account for 15 additional terminations. The two contigs differ in densities of ORFs, insertion elements and repeated sequences; together they contain two copies of the previously reported insertion sequence ISC1217, five additional IS elements representing four novel types, four classes of long non-IS repeated sequences, and numerous short, perfect repeats.
Keyword Biochemistry & Molecular Biology
Microbiology
Biochemistry & Molecular Biology
Microbiology
BIOCHEMISTRY & MOLECULAR BIOLOGY
MICROBIOLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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