Cloning and characterization of the nuclear gene and cDNAs for triosephosphate isomerase of the marine red alga Gracilaria verrucosa

Zhou Y.-H. and Ragan M.A. (1995) Cloning and characterization of the nuclear gene and cDNAs for triosephosphate isomerase of the marine red alga Gracilaria verrucosa. Current Genetics, 28 4: 317-323. doi:10.1007/BF00326429


Author Zhou Y.-H.
Ragan M.A.
Title Cloning and characterization of the nuclear gene and cDNAs for triosephosphate isomerase of the marine red alga Gracilaria verrucosa
Journal name Current Genetics   Check publisher's open access policy
ISSN 0172-8083
Publication date 1995-01-01
Year available 1995
Sub-type Article (original research)
DOI 10.1007/BF00326429
Open Access Status Not yet assessed
Volume 28
Issue 4
Start page 317
End page 323
Total pages 7
Place of publication NEW YORK
Publisher SPRINGER VERLAG
Language eng
Subject 1311 Genetics
Abstract cDNAs and an intronless single-copy nuclear gene (TPI1) encoding triosephosphate isomerase have been cloned and sequenced from the marine red alga Gracilaria verrucosa. The predicted amino-acid sequence of TPI1 is readily alignable with those of other known TPIs; 26 of 27 active-site residues and 19 of 26 intersubunit-contact residues are identical between TPIs of G. verrucosa and/or animals and green plants. A partial cDNA sequence of a second TPI gene (TPI2), presumably encoding plastid-localized TPI, was recovered by PCR and demonstrated by phylogenetic analysis to be red algal; no TPI2 cDNA or genomic clones could be recovered. Genomic Southern analysis demonstrated that at least two TPI-like genes are present in the nuclear DNA of G. verrucosa.
Keyword Gene structure
Gracilaria verrucosa
Triosephosphate isomerase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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