Characterization of a galactose-1-phosphate uridylyltransferase gene from the marine red alga Graeilaria gracilis

Lluisma A.O. and Ragan M.A. (1998) Characterization of a galactose-1-phosphate uridylyltransferase gene from the marine red alga Graeilaria gracilis. Current Genetics, 34 2: 112-119. doi:10.1007/s002940050374


Author Lluisma A.O.
Ragan M.A.
Title Characterization of a galactose-1-phosphate uridylyltransferase gene from the marine red alga Graeilaria gracilis
Journal name Current Genetics   Check publisher's open access policy
ISSN 0172-8083
Publication date 1998-01-01
Year available 1998
Sub-type Article (original research)
DOI 10.1007/s002940050374
Open Access Status Not yet assessed
Volume 34
Issue 2
Start page 112
End page 119
Total pages 8
Place of publication NEW YORK
Publisher SPRINGER VERLAG
Language eng
Subject 1311 Genetics
Abstract The metabolism of D-galactose is a major feature of red-algal physiology. We have cloned and sequenced a gene from the red alga Gracilaria gracilis that encodes a key enzyme of D-galactose metabolism, galactose-l-phosphate uridylyltransferase (GALT). This gene, designated GgGALT1, is apparently devoid of introns. A potential TATA box, four potential CAAT boxes, and a repeated sequence occur in the 5'-flanking region. The predicted 369-aa peptide shares significant sequence similarity with GALTs from other organisms (human, 47%; Saccharomyces cerevisiae, 49%; Solanum tuberosum, 49%). Southern-hybridization analysis reveals two related, but apparently not identical, GALT genes in the nuclear genome of G. gracilis. Sequence analysis indicates that the GgGALT1 enzyme lacks a rubredoxin "knuckle" motif, which in bacterial and fungal GALTs is involved in binding zinc. An open reading frame encoding a potential peptidyl tRNA hydrolase occurs 179 bp downstream from the GgGALT1 gene.
Keyword Galactose metabolism
Gracilaria gracilis
Nuclear gene
Rhodophyceae
Uridylyltransferase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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