Alterations of hippocampal glucose metabolism by even versus uneven medium chain triglycerides

McDonald, Tanya S., Tan, Kah Ni, Hodson, Mark P. and Borges, Karin (2014) Alterations of hippocampal glucose metabolism by even versus uneven medium chain triglycerides. Journal Of Cerebral Blood Flow and Metabolism, 34 1: 153-160. doi:10.1038/jcbfm.2013.184


Author McDonald, Tanya S.
Tan, Kah Ni
Hodson, Mark P.
Borges, Karin
Title Alterations of hippocampal glucose metabolism by even versus uneven medium chain triglycerides
Journal name Journal Of Cerebral Blood Flow and Metabolism   Check publisher's open access policy
ISSN 0271-678X
1559-7016
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1038/jcbfm.2013.184
Volume 34
Issue 1
Start page 153
End page 160
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher I O S Press
Language eng
Abstract Medium chain triglycerides (MCTs) are used to treat neurologic disorders with metabolic impairments, including childhood epilepsy and early Alzheimer’s disease. However, the metabolic effects of MCTs in the brain are still unclear. Here, we studied the effects of feeding even and uneven MCTs on brain glucose metabolism in the mouse. Adult mice were fed 35% (calories) of trioctanoin or triheptanoin (the triglycerides of octanoate or heptanoate, respectively) or a matching control diet for 3 weeks. Enzymatic assays and targeted metabolomics by liquid chromatography tandem mass spectrometry were used to quantify metabolites in extracts from the hippocampal formations (HFs). Both oils increased the levels of β-hydroxybutyrate, but no other significant metabolic alterations were observed after triheptanoin feeding. The levels of glucose 6-phosphate and fructose 6-phosphate were increased in the HF of mice fed trioctanoin, whereas levels of metabolites further downstream in the glycolytic pathway and the pentose phosphate pathway were reduced. This indicates that trioctanoin reduces glucose utilization because of a decrease in phosphofructokinase activity. Trioctanoin and triheptanoin showed similar anticonvulsant effects in the 6 Hz seizure model, but it remains unknown to what extent the anticonvulsant mechanism(s) are shared. In conclusion, triheptanoin unlike trioctanoin appears to not alter glucose metabolism in the healthy brain.
Keyword Epilepsy
Metabolomics
Mitochondria
Pentose phosphate pathway
Seizure
Tricarboxylic acid cycle
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 30 October 2013.

 
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Created: Wed, 22 Jan 2014, 19:47:19 EST by Dr Karin Borges on behalf of School of Biomedical Sciences