Extensive tRNA gene changes in synthetic Brassica napus

Wei, Lijuan, An, Zeshan, Mason, Annaliese S., Xiao, Meili, Chen, Fuyou, Guo, Ying, Li, Jiana and Fu, Donghui (2014) Extensive tRNA gene changes in synthetic Brassica napus. Journal of Molecular Evolution, 78 1: 38-49. doi:10.1007/s00239-013-9598-4

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Author Wei, Lijuan
An, Zeshan
Mason, Annaliese S.
Xiao, Meili
Chen, Fuyou
Guo, Ying
Li, Jiana
Fu, Donghui
Title Extensive tRNA gene changes in synthetic Brassica napus
Formatted title
Extensive tRNA gene changes in synthetic Brassica napus
Journal name Journal of Molecular Evolution   Check publisher's open access policy
ISSN 0022-2844
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s00239-013-9598-4
Open Access Status File (Author Post-print)
Volume 78
Issue 1
Start page 38
End page 49
Total pages 12
Place of publication New York, United States
Publisher Springer
Language eng
Formatted abstract
Allopolyploidization, where two species come together to form a new species, plays a major role in speciation and genome evolution. Transfer RNAs (abbreviated tRNA) are typically 73–94 nucleotides in length, and are indispensable in protein synthesis, transferring amino acids to the cell protein synthesis machinery (ribosome). To date, the regularity and function of tRNA gene sequence variation during the process of allopolyploidization have not been well understood. In this study, the inter-tRNA gene corresponding to tRNA amplification polymorphism method was used to detect changes in tRNA gene sequences in the progeny of interspecific hybrids between Brassica rapa and B. oleracea, mimicking the original B. napus (canola) species formation event. Cluster analysis showed that tRNA gene variation during allopolyploidization did not appear to have a genotypic basis. Significant variation occurred in the early generations of synthetic B. napus (F1 and F2 generations), but fewer alterations were observed in the later generation (F3). The variation-prone tRNA genes tended to be located in AT-rich regions. BlastN analysis of novel tRNA gene variants against a Brassica genome sequence database showed that the variation of these tRNA-gene-associated sequences in allopolyploidization might result in variation of gene structure and function, e.g., metabolic process and transport.
Keyword Brassica napus
tRNA gene
Sequence variation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 23 November 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2014 Collection
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Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
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Created: Thu, 16 Jan 2014, 21:19:44 EST by Annaliese Mason on behalf of School of Agriculture and Food Sciences