Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids

Bonning, Bryony C., Pal, Narinder, Liu, Sijun, Wang, Zhaohui, Sivakumar, S., Dixon, Philip M., King, Glenn F. and Miller, W. Allen (2014) Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids. Nature Biotechnology, 32 1: 102-105. doi:10.1038/nbt.2753


Author Bonning, Bryony C.
Pal, Narinder
Liu, Sijun
Wang, Zhaohui
Sivakumar, S.
Dixon, Philip M.
King, Glenn F.
Miller, W. Allen
Title Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids
Journal name Nature Biotechnology   Check publisher's open access policy
ISSN 1087-0156
1546-1696
Publication date 2014-01-01
Year available 2013
Sub-type Letter to editor, brief commentary or brief communication
DOI 10.1038/nbt.2753
Open Access Status Not Open Access
Volume 32
Issue 1
Start page 102
End page 105
Total pages 4
Place of publication New York, NY, United States
Publisher Nature Publishing Group
Language eng
Formatted abstract
The sap-sucking insects (order Hemiptera), including aphids, planthoppers, whiteflies and stink bugs, present one of the greatest challenges for pest management in global agriculture. Insect neurotoxins offer an alternative to chemical insecticides for controlling these pests, but require delivery into the insect hemocoel. Here we use the coat protein of a luteovirus, an aphid-vectored plant virus, to deliver a spider-derived, insect-specific toxin that acts within the hemocoel. The luteovirid coat protein is sufficient for delivery of fused proteins into the hemocoel of pea aphids, Acyrthosiphon pisum, without virion assembly. We show that when four aphid pest species—A. pisum, Rhopalosiphum padi, Aphis glycines and Myzus persicae—feed on a recombinant coat protein–toxin fusion, either in an experimental membrane sachet or in transgenic Arabidopsis plants, they experience significant mortality. Aphids fed on these fusion proteins showed signs of neurotoxin-induced paralysis. Luteovirid coat protein–insect neurotoxin fusions represent a promising strategy for transgenic control of aphids and potentially other hemipteran pests.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 8 December 2013

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 21 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 16 Jan 2014, 01:23:15 EST by Susan Allen on behalf of Institute for Molecular Bioscience