Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix

Grewal, Yadveer S., Shiddiky, Muhammad J. A., Spadafora, Lauren J., Cangelosi, Gerard A. and Trau, Matt (2014) Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix. Biosensors and Bioelectronics, 55 417-422. doi:10.1016/j.bios.2013.12.043


Author Grewal, Yadveer S.
Shiddiky, Muhammad J. A.
Spadafora, Lauren J.
Cangelosi, Gerard A.
Trau, Matt
Title Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix
Formatted title
Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2014-05-15
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.bios.2013.12.043
Volume 55
Start page 417
End page 422
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Highlights
• First demonstration of nano-yeast–scFv based immunosensor on a portable-low cost platform.
• New antibody/antigen combination demonstrates that nano-yeast–scFv can be routinely engineered towards diverse target antigens.
• Nano-yeast–scFv based immunosensor was successfully used for E. histolytica antigen detection in a biological matrix derived from human stool.

The time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. Herein, we present low-cost affinity reagents, nano-yeast–scFv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free electrochemical detection of Entamoeba histolytica cyst antigens. The biosensor was able to detect antigen at concentrations down to 10 pg mL−1 in buffer with an inter-assay reproducibility of (% RSD, n=3) 4.1%. The applicability of two differently engineered nano-yeast–scFv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biological matrix derived from human stool. Because of the simple, inexpensive, and sensitive nature of this methodology, it may offer a low-cost alternative to immunosensors based on antibody-target recognition.
Keyword Nano-yeast–scFv
Affinity reagent
Electrochemical immunosensor
Screen-printed electrode
Entamoeba histolytica
Label-free detection
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 27 December 2013

 
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Created: Wed, 15 Jan 2014, 21:32:48 EST by Yadveer Singh Grewal on behalf of Aust Institute for Bioengineering & Nanotechnology