Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix

Grewal, Yadveer S., Shiddiky, Muhammad J. A., Spadafora, Lauren J., Cangelosi, Gerard A. and Trau, Matt (2014) Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix. Biosensors and Bioelectronics, 55 417-422. doi:10.1016/j.bios.2013.12.043


Author Grewal, Yadveer S.
Shiddiky, Muhammad J. A.
Spadafora, Lauren J.
Cangelosi, Gerard A.
Trau, Matt
Title Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix
Formatted title
Nano-yeast–scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2014-05-15
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.bios.2013.12.043
Open Access Status Not yet assessed
Volume 55
Start page 417
End page 422
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 1305 Biotechnology
1304 Biophysics
2204 Biomedical Engineering
1603 Electrochemistry
Abstract The time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. Herein, we present low-cost affinity reagents, nano-yeast-scFv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free electrochemical detection of Entamoeba histolytica cyst antigens. The biosensor was able to detect antigen at concentrations down to 10pgmL in buffer with an inter-assay reproducibility of (% RSD, n=3) 4.1%. The applicability of two differently engineered nano-yeast-scFv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biological matrix derived from human stool. Because of the simple, inexpensive, and sensitive nature of this methodology, it may offer a low-cost alternative to immunosensors based on antibody-target recognition.
Formatted abstract
Highlights
• First demonstration of nano-yeast–scFv based immunosensor on a portable-low cost platform.
• New antibody/antigen combination demonstrates that nano-yeast–scFv can be routinely engineered towards diverse target antigens.
• Nano-yeast–scFv based immunosensor was successfully used for E. histolytica antigen detection in a biological matrix derived from human stool.

The time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. Herein, we present low-cost affinity reagents, nano-yeast–scFv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free electrochemical detection of Entamoeba histolytica cyst antigens. The biosensor was able to detect antigen at concentrations down to 10 pg mL−1 in buffer with an inter-assay reproducibility of (% RSD, n=3) 4.1%. The applicability of two differently engineered nano-yeast–scFv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biological matrix derived from human stool. Because of the simple, inexpensive, and sensitive nature of this methodology, it may offer a low-cost alternative to immunosensors based on antibody-target recognition.
Keyword Nano-yeast–scFv
Affinity reagent
Electrochemical immunosensor
Screen-printed electrode
Entamoeba histolytica
Label-free detection
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID U01AIO82186-01
Institutional Status UQ
Additional Notes Available online 27 December 2013

 
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Created: Wed, 15 Jan 2014, 21:32:48 EST by Yadveer Singh Grewal on behalf of Aust Institute for Bioengineering & Nanotechnology