Exosomal and non-exosomal transport of extra-cellular microRNAs in follicular fluid: implications for bovine oocyte developmental competence

Sohel, Md. Mahmodul Hasan, Hoelker, Michael, Noferesti, Sina Seifi, Salilew-Wondim, Dessie, Tholen, Ernst, Looft, Christian, Rings, Franca, Uddin, Muhammad Jasim, Spencer, Thomas E., Schellander, Karl and Tesfaye, Dawit (2013) Exosomal and non-exosomal transport of extra-cellular microRNAs in follicular fluid: implications for bovine oocyte developmental competence. PLoS One, 8 11: e78505.1-e78505.16. doi:10.1371/journal.pone.0078505


Author Sohel, Md. Mahmodul Hasan
Hoelker, Michael
Noferesti, Sina Seifi
Salilew-Wondim, Dessie
Tholen, Ernst
Looft, Christian
Rings, Franca
Uddin, Muhammad Jasim
Spencer, Thomas E.
Schellander, Karl
Tesfaye, Dawit
Title Exosomal and non-exosomal transport of extra-cellular microRNAs in follicular fluid: implications for bovine oocyte developmental competence
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-11-01
Year available 2013
Sub-type Article (original research)
DOI 10.1371/journal.pone.0078505
Open Access Status DOI
Volume 8
Issue 11
Start page e78505.1
End page e78505.16
Total pages 16
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract Cell-cell communication within the follicle involves many signaling molecules, and this process may be mediated by secretion and uptake of exosomes that contain several bioactive molecules including extra-cellular miRNAs. Follicular fluid and cells from individual follicles of cattle were grouped based on Brilliant Cresyl Blue (BCB) staining of the corresponding oocytes. Both Exoquick precipitation and differential ultracentrifugation were used to separate the exosome and non-exosomal fraction of follicular fluid. Following miRNA isolation from both fractions, the human miRCURY LNA™ Universal RT miRNA PCR array system was used to profile miRNA expression. This analysis found that miRNAs were present in both exosomal and non-exosomal fraction of bovine follicular fluid. We found 25 miRNAs differentially expressed (16 up and 9 down) in exosomes and 30 miRNAs differentially expressed (21 up and 9 down) in non-exosomal fraction of follicular fluid in comparison of BCB- versus BCB+ oocyte groups. Expression of selected miRNAs was detected in theca, granulosa and cumulus oocyte complex. To further explore the potential roles of these follicular fluid derived extra-cellular miRNAs, the potential target genes were predicted, and functional annotation and pathway analysis revealed most of these pathways are known regulators of follicular development and oocyte growth. In order to validate exosome mediated cell-cell communication within follicular microenvironment, we demonstrated uptake of exosomes and resulting increase of endogenous miRNA level and subsequent alteration of mRNA levels in follicular cells in vitro. This study demonstrates for the first time, the presence of exosome or non-exosome mediated transfer of miRNA in the bovine follicular fluid, and oocyte growth dependent variation in extra-cellular miRNA signatures in the follicular environment.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
MULTIDISCIPLINARY SCIENCES
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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