Detecting, visualising, and quantifying mucins

Harrop, Ceri A., Thornton, David J. and McGuckin, Michael A. (2012). Detecting, visualising, and quantifying mucins. In Michael A. McGuckin and David J. Thornton (Ed.), Mucins: Methods and Protocols (pp. 49-66) New York, NY, United States: Humana Press. doi:10.1007/978-1-61779-513-8_3


Author Harrop, Ceri A.
Thornton, David J.
McGuckin, Michael A.
Title of chapter Detecting, visualising, and quantifying mucins
Title of book Mucins: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2012
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-61779-513-8_3
Open Access Status Not Open Access
Series Methods in Molecular Biology
ISBN 9781617795121
9781617795138
ISSN 1064-3745
1940-6029
Editor Michael A. McGuckin
David J. Thornton
Volume number 842
Chapter number 3
Start page 49
End page 66
Total pages 18
Total chapters 19
Formatted Abstract/Summary
The extreme size, extensive glycosylation, and gel-forming nature of mucins make them a challenge to work with, and methodologies for the detection of mucins must take into consideration these features to ensure that one obtains both accurate and meaningful results. In understanding and appreciating the nature of mucins, this affords the researcher a valuable toolkit which can be used to full advantage in detecting, quantifying, and visualising mucins. The employment of a combinatorial approach to mucin detection, using antibody, chemical, and lectin detection methods, allows important information to be gleaned regarding the size, extent of glycosylation, specific mucin species, and distribution of mucins within a given sample. In this chapter, the researcher is guided through considerations into the structure of mucins and how this both affects the detection of mucins and can be used to full advantage. Techniques including ELISA, dot/slot blotting, and Western blotting, use of lectins and antibodies in mucin detection on membranes as well as immunohistochemistry and immunofluorescence on both tissues and cells grown on Transwell™ inserts are described. Notes along with each section advice the researcher on best practice and describe any associated limitations of a particular technique from which the researcher can further develop a particular protocol.
Keyword Mucin
Detection
ELISA
Western blotting
Immunohistochemistry
Antibody
Lectin
Bronchial epithelial cell culture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 15 Jan 2014, 04:01:02 EST by Michael Mcguckin on behalf of School of Biomedical Sciences