Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers

Hartono, Sandy B., Yu, Meihua, Gu, Wenyi, Yang, Jie, Strounina, Ekaterina, Wang, Xiaolin, Qiao, Shizhang and Yu, Chengzhong (2014) Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers. Nanotechnology, 25 5: 055701.1-055701.13. doi:10.1088/0957-4484/25/5/055701


Author Hartono, Sandy B.
Yu, Meihua
Gu, Wenyi
Yang, Jie
Strounina, Ekaterina
Wang, Xiaolin
Qiao, Shizhang
Yu, Chengzhong
Title Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers
Journal name Nanotechnology   Check publisher's open access policy
ISSN 0957-4484
1361-6528
Publication date 2014-02-07
Year available 2014
Sub-type Article (original research)
DOI 10.1088/0957-4484/25/5/055701
Open Access Status Not yet assessed
Volume 25
Issue 5
Start page 055701.1
End page 055701.13
Total pages 13
Place of publication Temple Way, Bristol, United Kingdom
Publisher Institute of Physics Publishing
Language eng
Abstract The development of functional nanocarriers that can enhance the cellular delivery of a variety of nucleic acid agents is important in many biomedical applications such as siRNA therapy. We report the synthesis of large pore mesoporous silica nanoparticles (LPMSN) loaded with iron oxide and covalently modified by polyethyleneimine (denoted PEI-Fe-LPMSN) as carriers for gene delivery. The LPMSN have a particle size of similar to 200 nm and a large pore size of 11 nm. The large pore size is essential for the formation of large iron oxide nanoparticles to increase the magnetic properties and the adsorption capacity of siRNA molecules. The magnetic property facilitates the cellular uptake of nanocarriers under an external magnetic field. PEI is covalently grafted on the silica surface to enhance the nanocarriers' affinity against siRNA molecules and to improve gene silencing performance. The PEI-Fe-LPMSN delivered siRNA-PLK1 effectively into osteosarcoma cancer cells, leading to cell viability inhibition of 80%, higher compared to the 50% reduction when the same dose of siRNA was delivered by a commercial product, oligofectamine.
Formatted abstract
The development of functional nanocarriers that can enhance the cellular delivery of a variety of nucleic acid agents is important in many biomedical applications such as siRNA therapy. We report the synthesis of large pore mesoporous silica nanoparticles (LPMSN) loaded with iron oxide and covalently modified by polyethyleneimine (denoted PEI-Fe-LPMSN) as carriers for gene delivery. The LPMSN have a particle size of ~200 nm and a large pore size of 11 nm. The large pore size is essential for the formation of large iron oxide nanoparticles to increase the magnetic properties and the adsorption capacity of siRNA molecules. The magnetic property facilitates the cellular uptake of nanocarriers under an external magnetic field. PEI is covalently grafted on the silica surface to enhance the nanocarriers' affinity against siRNA molecules and to improve gene silencing performance. The PEI-Fe-LPMSN delivered siRNA–PLK1 effectively into osteosarcoma cancer cells, leading to cell viability inhibition of 80%, higher compared to the 50% reduction when the same dose of siRNA was delivered by a commercial product, oligofectamine.
Keyword Nanomaterials
Gene delivery
siRNA
Polyethyleneimine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 15 Jan 2014, 00:20:51 EST by Cathy Fouhy on behalf of Functional Nanomaterials