Influence of novel nano-mesoporous bioactive glass on the regulation of IGF-II gene expression in osteoblasts

Quan, Zhang, Han, Xie, Ye, Zhang, Chenzhong, Yu and Wenjun, Chen (2012) Influence of novel nano-mesoporous bioactive glass on the regulation of IGF-II gene expression in osteoblasts. Cell Biochemistry and Biophysics, 62 1: 119-123. doi:10.1007/s12013-011-9269-2


Author Quan, Zhang
Han, Xie
Ye, Zhang
Chenzhong, Yu
Wenjun, Chen
Title Influence of novel nano-mesoporous bioactive glass on the regulation of IGF-II gene expression in osteoblasts
Journal name Cell Biochemistry and Biophysics   Check publisher's open access policy
ISSN 1085-9195
1559-0283
Publication date 2012-01-01
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s12013-011-9269-2
Open Access Status Not Open Access
Volume 62
Issue 1
Start page 119
End page 123
Total pages 15
Place of publication Totowa, NJ, United States
Publisher Humana Press
Language eng
Abstract To investigate whether the mesoporous bioactive glass (MBG) exerts any in vitro bioactivity on rat osteoblasts and the potential regulatory mechanism of this bioactivity. Rat osteoblasts were incubated in the presence and absence (control) of ionic dissolution product of MBG in minimal essential medium. The osteoblast proliferation and differentiation were measured using MTT and alkaline phosphatase methods. The IGF-II mRNA expression in osteoblasts was measured by RT-PCR. IGF-II protein and IGFBP were measured by ELISA. The level of alkaline phosphatase activity was increased to 125% of control. Expression of IGF-II mRNA was increased to 125% of control. There was a 175 and 237% increase in the concentration of unbound IGF-II protein and IGFBP, respectively, in the conditioned media of treated osteoblasts. The osteoblast proliferation was 92% of control. The ionic dissolution product of MBG was able to promote the differentiation of osteoblasts, probably by inducing IGF-II expression at both mRNA and protein level.
Keyword Bioactive glass
Differentiation
Gene regulation
IGF-II
Nano-mesoporous
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes http://www.ncbi.nlm.nih.gov/pubmed/21858714

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 14 Jan 2014, 22:12:06 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology