The antiangiogenic activities of ethanolic crude extracts of four Salvia species

Zihlif M., Afifi F., Abu-Dahab R., Abdul Majid A.M.S., Somrain H., Saleh M.M., Nassar Z.D. and Naffa R. (2013) The antiangiogenic activities of ethanolic crude extracts of four Salvia species. BMC Complementary and Alternative Medicine, 13 358.1-358.10. doi:10.1186/1472-6882-13-358

Author Zihlif M.
Afifi F.
Abu-Dahab R.
Abdul Majid A.M.S.
Somrain H.
Saleh M.M.
Nassar Z.D.
Naffa R.
Title The antiangiogenic activities of ethanolic crude extracts of four Salvia species
Journal name BMC Complementary and Alternative Medicine   Check publisher's open access policy
ISSN 1472-6882
Publication date 2013-12-13
Year available 2013
Sub-type Article (original research)
DOI 10.1186/1472-6882-13-358
Open Access Status DOI
Volume 13
Start page 358.1
End page 358.10
Total pages 10
Place of publication London United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Angiogenesis is one of cancer hallmarks that are required for both cancer progression and metastasis. In this study we examined the antiangiogenic properties of the ethanolic crude extracts of four Salvia species grown in Jordan.

Methods: The direct antiangiogenic activity was evaluated using various models: ex vivo rat aortic ring assay, in vitro assessment of HUVEC proliferation and migration, and in vivo CAM assay, while we used the changes in the expression of HIF-1α and VEGF in breast cancer cells (MCF 7) as an indicative for the indirect antiangiogenic activity.

Results: All four crude extracts showed a potential antiangiogenic activity in the rat aortic assay, however two species were found to be cytotoxic against Fibroblast cell line (PLF); the finding that caused the exclusion of these two extracts from further studies. Of the two remaining extracts, S. triloba showed very promising direct and indirect antiangiogenic activities. S. triloba inhibited the HUVEC proliferation with an IC50 of 90 μg/mL and HUVEC migration by 82% at 150 μg/mL. Furthermore, the in vivo CAM assay also illustrated the high impact of S. triloba against the newly formed vessel in the chicken embryonic membrane. Interestingly, the S. triloba inhibited the expression of VEGF at the mRNA and protein and the HIF-1α mRNA in the MCF 7 breast cancer cells under both normoxic and hypoxic conditions.

Conclusions: Taken together, all these findings of the direct and indirect angiogenic investigations nominated S. triloba as a highly potent antiangiogenic plant that may have chemotherapeutic and/or chemoprevention potentials.
Keyword Antiangiogenesis
Salvia dominica
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Pharmacy Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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