Optimized method for quantification of total F2-isoprostanes using gas chromatography–tandem mass spectrometry

Briskey, David R., Wilson, Gary R., Fassett, Robert G. and Coombes, Jeff S. (2014) Optimized method for quantification of total F2-isoprostanes using gas chromatography–tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 90 161-166. doi:10.1016/j.jpba.2013.11.028


Author Briskey, David R.
Wilson, Gary R.
Fassett, Robert G.
Coombes, Jeff S.
Title Optimized method for quantification of total F2-isoprostanes using gas chromatography–tandem mass spectrometry
Journal name Journal of Pharmaceutical and Biomedical Analysis   Check publisher's open access policy
ISSN 0731-7085
1873-264X
Publication date 2014-03-05
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jpba.2013.11.028
Volume 90
Start page 161
End page 166
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
F2-isoprostanes are produced from the oxidative degradation of arachidonic acid and are considered the gold standard marker of lipid peroxidation in biological samples. We developed a liquid–liquid extraction method for the determination of total isoprostanes using negative chemical ionization gas chromatography–tandem mass spectrometry in plasma and tissue homogenates. Incorporating liquid–liquid extraction allows for greater sample through-put than current approaches. Here we describe the protocol and include numerous trouble-shooting suggestions. The method found healthy individuals with 150–250 pg of isoprostanes per ml of plasma and end stage kidney disease patients to have the highest measured values of up to 1100 pg/ml. This assay has an accurate working linear range of 40–1000 pg of isoprostanes (100–2500 pg/ml) and an average coefficient of variance of 7%. Tissue values for healthy mice liver were 50–70 pg/μg protein. This method provides increased ion selectivity and detection capabilities with economical sample through-put.
Keyword Gas chromatography
Tandem mass spectrometry
Oxidative stress
Lipid peroxidation
Liquid–liquid extraction
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 1 December 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Human Movement and Nutrition Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 13 Jan 2014, 04:23:40 EST by Deborah Noon on behalf of School of Human Movement and Nutrition Sciences