Host defence peptide LL-37 induces IL-6 expression in human bronchial epithelial cells by activation of the NF-κb signaling pathway

Pistolic, Jelena, Cosseau, Celine, Li, Yuexin, Yu, Jie (Jessie), Filewod, Niall C. J., Gellatly, Shaan, Rehaume, Linda M., Bowdish, Dawn M. E. and Hancock, Robert E. W. (2009) Host defence peptide LL-37 induces IL-6 expression in human bronchial epithelial cells by activation of the NF-κb signaling pathway. Journal of Innate Immunity, 1 3: 254-267. doi:10.1159/000171533


Author Pistolic, Jelena
Cosseau, Celine
Li, Yuexin
Yu, Jie (Jessie)
Filewod, Niall C. J.
Gellatly, Shaan
Rehaume, Linda M.
Bowdish, Dawn M. E.
Hancock, Robert E. W.
Title Host defence peptide LL-37 induces IL-6 expression in human bronchial epithelial cells by activation of the NF-κb signaling pathway
Journal name Journal of Innate Immunity   Check publisher's open access policy
ISSN 1662-811X
1662-8128
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1159/000171533
Open Access Status Not Open Access
Volume 1
Issue 3
Start page 254
End page 267
Total pages 14
Place of publication Basel, Switzerland
Publisher S. Karger
Language eng
Abstract LL-37, the only member of the cathelicidin family of cationic host defence peptides in humans, has been shown to mediate multiple immunomodulatory effects and as such is thought to be an important component of innate immune responses. A growing body of evidence indicates that LL-37 affects lung mucosal responses to pathogens through altered regulation of cell migration, proliferation, wound healing and cell apoptosis. These functions are consistent with LL-37 playing a role in regulating lung epithelial inflammatory responses; however, that role has not been clearly defined. In this report we have demonstrated that host defence peptide LL-37 induced cytokine (IL-6) and chemokine (CXCL-1/GRO-α and CXCL-8/IL-8) release from human bronchial epithelial cells. It was demonstrated that LL-37-mediated IL-6 release was time and dose dependent and that LL-37 up-regulated this pleiotropic cytokine at the transcriptional level. Using specific inhibitors it was shown that NF-κB signaling led to the LL-37-stimulated production of IL-6. LL-37 stimulation of airway epithelial cells activated NF-κB signaling, as demonstrated by the phosphorylation and degradation of Iκ-Bα, and consequent nuclear translocation of p65 and p50 NF-κB subunits. Furthermore this host defence peptide augmented flagellin-mediated cytokine production, indicating that LL-37 likely modulates Toll-like receptor 5-mediated responses.
Keyword Bronchial epithelial cells
IL-6
LL-37
NF-κB
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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