Visualization of mouse barrel cortex using ex-vivo track density imaging

Kurniawan, Nyoman D., Richards, Kay L., Yang, Zhengyi, She, David, Ullmann, Jeremy F. P., Moldrich, Randal X., Liu, Sha, Yaksic, Javier Urriola, Leanage, Gayeshika, Kharatishvilia, Irina, Wimmer, Verena, Calamante, Fernando, Galloway, Graham J., Petrou, Steven and Reutens, David C. (2014) Visualization of mouse barrel cortex using ex-vivo track density imaging. NeuroImage, 87 465-475. doi:10.1016/j.neuroimage.2013.09.030

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Author Kurniawan, Nyoman D.
Richards, Kay L.
Yang, Zhengyi
She, David
Ullmann, Jeremy F. P.
Moldrich, Randal X.
Liu, Sha
Yaksic, Javier Urriola
Leanage, Gayeshika
Kharatishvilia, Irina
Wimmer, Verena
Calamante, Fernando
Galloway, Graham J.
Petrou, Steven
Reutens, David C.
Title Visualization of mouse barrel cortex using ex-vivo track density imaging
Journal name NeuroImage   Check publisher's open access policy
ISSN 1053-8119
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.neuroimage.2013.09.030
Open Access Status File (Author Post-print)
Volume 87
Start page 465
End page 475
Total pages 11
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
We describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adultmouse brainwith short-tracks track density imaging (stTDI). stTDI producedmuch higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FAmaps, spin-echo T1- andT2-weighted imaging and gradient echo T1/T2*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48 micron isotropic resolution for a (3 mm)3 block of cortex containing the barrel field and reconstructed using stTDI at 10 micron isotropic resolution. HARDI datawere also acquired at 100 micron isotropic resolution to image the whole brain and reconstructed using stTDI at 20 micron isotropic resolution. The 10 micron resolution stTDI maps showed exceptionally clear delineation of barrel structures. Individual barrels could also be distinguished in the 20 micron stTDI maps but the septa separating the individual barrels appeared thicker compared to the 10 micron maps, indicating that the ability of stTDI to produce high quality structural delineation is dependent upon acquisition resolution. Close homologywas observed between the barrel structure delineated using stTDI and reconstructed histological data from the same samples. stTDI also detects barrel
deletions in the posterior medial barrel sub-field in mice with infraorbital nerve cuts. The results demonstrate that stTDI is a novel imaging technique that enables three-dimensional characterization of complex structures such as the barrels in S1 and provides an important complementary non-invasive imaging tool for studying synaptic connectivity, development and plasticity of the sensory system.
Keyword Mouse Brain
Barrel Cortex
Magnetic Resonance (mr)
Diffusion weighted imaging
Track-density imaging
Infraorbital nerve cut
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 21 September 2013.

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Created: Fri, 10 Jan 2014, 00:40:53 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging