Allantoin catabolism influences the production of antibiotics in Streptomyces coelicolor

Navone, Laura, Casati, Paula, Licona-Cassani, Cuauhtémoc, Marcellin, Esteban, Nielsen, Lars K., Rodriguez, Eduardo and Gramajo, Hugo (2014) Allantoin catabolism influences the production of antibiotics in Streptomyces coelicolor. Applied Microbiology and Biotechnology., 98 1: 351-360. doi:10.1007/s00253-013-5372-1


Author Navone, Laura
Casati, Paula
Licona-Cassani, Cuauhtémoc
Marcellin, Esteban
Nielsen, Lars K.
Rodriguez, Eduardo
Gramajo, Hugo
Title Allantoin catabolism influences the production of antibiotics in Streptomyces coelicolor
Formatted title
Allantoin catabolism influences the production of antibiotics in Streptomyces coelicolor
Journal name Applied Microbiology and Biotechnology.   Check publisher's open access policy
ISSN 0175-7598
1432-0614
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s00253-013-5372-1
Volume 98
Issue 1
Start page 351
End page 360
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Purines are a primary source of carbon and nitrogen in soil; however, their metabolism is poorly understood in Streptomyces. Using a combination of proteomics, metabolomics, and metabolic engineering, we characterized the allantoin pathway in Streptomyces coelicolor. When cells grew in glucose minimal medium with allantoin as the sole nitrogen source, quantitative proteomics identified 38 enzymes upregulated and 28 downregulated. This allowed identifying six new functional enzymes involved in allantoin metabolism in S. coelicolor. From those, using a combination of biochemical and genetic engineering tools, it was found that allantoinase (EC 3.5.2.5) and allantoicase (EC 3.5.3.4) are essential for allantoin metabolism in S. coelicolor. Metabolomics showed that under these growth conditions, there is a significant intracellular accumulation of urea and amino acids, which eventually results in urea and ammonium release into the culture medium. Antibiotic production of a urease mutant strain showed that the catabolism of allantoin, and the subsequent release of ammonium, inhibits antibiotic production. These observations link the antibiotic production impairment with an imbalance in nitrogen metabolism and provide the first evidence of an interaction between purine metabolism and antibiotic biosynthesis.
Keyword Streptomyces
Allantoin
Ammonium
Antibiotic regulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 29 November 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 08 Jan 2014, 19:46:23 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology