Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells

Ross, Diana G. F., Smart, Chanel E., Azimi, Iman, Roberts-Thomson, Sarah J. and Monteith, Gregory R. (2013) Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells. BMC Cell Biology, 14 1: 57.1-57.10. doi:10.1186/1471-2121-14-57


Author Ross, Diana G. F.
Smart, Chanel E.
Azimi, Iman
Roberts-Thomson, Sarah J.
Monteith, Gregory R.
Title Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells
Journal name BMC Cell Biology   Check publisher's open access policy
ISSN 1471-2121
Publication date 2013-12-20
Year available 2013
Sub-type Article (original research)
DOI 10.1186/1471-2121-14-57
Open Access Status DOI
Volume 14
Issue 1
Start page 57.1
End page 57.10
Total pages 10
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Subject 1307 Cell Biology
Abstract Background: The entry of calcium ions into mammary gland epithelial cells is one of the least well-understood processes in the transport of calcium into milk during lactation. The store-operated calcium entry channel ORAI1, has been suggested as a potential mechanism for the entry of Ca2+ into mammary gland epithelial cells from the maternal blood supply during lactation. The down regulation of the canonical ORAI1 activator STIM1 during lactation suggests that other known ORAI activators such as STIM2 and SPCA2 may be important during lactation.
Formatted abstract
Background: The entry of calcium ions into mammary gland epithelial cells is one of the least well-understood processes in the transport of calcium into milk during lactation. The store-operated calcium entry channel ORAI1, has been suggested as a potential mechanism for the entry of Ca2+ into mammary gland epithelial cells from the maternal blood supply during lactation. The down regulation of the canonical ORAI1 activator STIM1 during lactation suggests that other known ORAI activators such as STIM2 and SPCA2 may be important during lactation.

Results: Differentiation of HC11 mammary gland epithelial cells was associated with enhanced basal Ca2+ influx. Silencing of Orai1 abolished this enhancement of Ca2+ influx. Stim2 had a modest effect on Ca2+ influx in this in vitro model of lactation, whereas Stim1 and Spca2 silencing had no effect. Despite pronounced increases in Spca2 mRNA during lactation there was no change in the generation of the alternative splice product generated by Mist1, which increases during lactation.

Conclusions: These studies support the hypothesis that lactation is associated with a remodelling of Ca2+ influx and this is associated with enhancement of basal Ca2+ influx. This enhanced Ca2+ influx appears to occur through the calcium channel Orai1.
Keyword Cell Biology
Cell Biology
CELL BIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 631347
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Pharmacy Publications
 
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