Quantifying adhesion of acidophilic bioleaching bacteria to silica and pyrite by atomic force microscopy with a bacterial probe

Diao, Mengxue, Taran, Elena, Mahler, Stephen, Nguyen, Tuan A. H. and Nguyen, Anh V. (2014) Quantifying adhesion of acidophilic bioleaching bacteria to silica and pyrite by atomic force microscopy with a bacterial probe. Colloids and Surfaces B: Biointerfaces, 115 229-236. doi:10.1016/j.colsurfb.2013.11.047


Author Diao, Mengxue
Taran, Elena
Mahler, Stephen
Nguyen, Tuan A. H.
Nguyen, Anh V.
Title Quantifying adhesion of acidophilic bioleaching bacteria to silica and pyrite by atomic force microscopy with a bacterial probe
Journal name Colloids and Surfaces B: Biointerfaces   Check publisher's open access policy
ISSN 0927-7765
1873-4367
Publication date 2014-03-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.colsurfb.2013.11.047
Open Access Status Not yet assessed
Volume 115
Start page 229
End page 236
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract The adhesion of acidophilic bacteria to mineral surfaces is an important phenomenon in bioleaching processes. In this study, functionalized colloidal probes covered by bioleaching bacterial cells (Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans) were developed and used to sense specific adhesion forces to a silica surface and a pyrite surface in various solutions. Experimentally, recorded retraction curves of A. thiooxidans revealed sawtooth features that were in good agreement with the wormlike chain model, while that of L. ferrooxidans exhibited stair-step separation. The magnitudes of adhesion forces and snap-off distances were strongly influenced by the ionic strength and pH. Macroscopic surface properties including hydrophobicity and surface potential for bacterial cells and substrata were measured by a sessile drop method and microelectrophoresis. The ATR-FTIR spectra indicated the presence of different types of biopolymers on two strains of bacteria. (C) 2014 Elsevier B.V. All rights reserved.
Formatted abstract
The adhesion of acidophilic bacteria to mineral surfaces is an important phenomenon in bioleaching processes. In this study, functionalized colloidal probes covered by bioleaching bacterial cells (Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans) were developed and used to sense specific adhesion forces to a silica surface and a pyrite surface in various solutions. Experimentally, recorded retraction curves of A. thiooxidans revealed sawtooth features that were in good agreement with the wormlike chain model, while that of L. ferrooxidans exhibited stair-step separation. The magnitudes of adhesion forces and snap-off distances were strongly influenced by the ionic strength and pH. Macroscopic surface properties including hydrophobicity and surface potential for bacterial cells and substrata were measured by a sessile drop method and microelectrophoresis. The ATR-FTIR spectra indicated the presence of different types of biopolymers on two strains of bacteria.
Keyword Acidithiobacillus thiooxidans
Adhesion force
Atomic force microscopy
Leptospirillum ferrooxidans
Pyrite
Snap-off distance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 1 December 2013.

 
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