Molecular nanoshearing: an innovative approach to shear off molecules with AC-induced nanoscopic fluid flow

Shiddiky, Muhammad J. A., Vaidyanathan, Ramanathan, Rauf, Sakandar, Tay, Zhikay and Trau, Matt (2014) Molecular nanoshearing: an innovative approach to shear off molecules with AC-induced nanoscopic fluid flow. Scientific Reports, 4 3716.1-3716.7. doi:10.1038/srep03716


Author Shiddiky, Muhammad J. A.
Vaidyanathan, Ramanathan
Rauf, Sakandar
Tay, Zhikay
Trau, Matt
Title Molecular nanoshearing: an innovative approach to shear off molecules with AC-induced nanoscopic fluid flow
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2014-01-16
Year available 2014
Sub-type Article (original research)
DOI 10.1038/srep03716
Open Access Status DOI
Volume 4
Start page 3716.1
End page 3716.7
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1000 General
Abstract Early diagnosis of disease requires highly specific measurement of molecular biomarkers from femto to pico-molar concentrations in complex biological (e.g., serum, blood, etc.) samples to provide clinically useful information. While reaching this detection limit is challenging in itself, these samples contain numerous other non-target molecules, most of which have a tendency to adhere to solid surfaces via nonspecific interactions. Herein, we present an entirely new methodology to physically displace nonspecifically bound molecules from solid surfaces by utilizing a newly discovered "tuneable force", induced by an applied alternating electric field, which occurs within few nanometers of an electrode surface. This methodology thus offers a unique ability to shear-off loosely bound molecules from the solid/liquid interface. Via this approach, we achieved a 5-fold reduction in nonspecific adsorption of non-target protein molecules and a 1000-fold enhancement for the specific capture of HER2 protein in human serum.
Formatted abstract
Early diagnosis of disease requires highly specific measurement of molecular biomarkers from femto to pico-molar concentrations in complex biological (e.g., serum, blood, etc.) samples to provide clinically useful information. While reaching this detection limit is challenging in itself, these samples contain numerous other non-target molecules, most of which have a tendency to adhere to solid surfaces via nonspecific interactions. Herein, we present an entirely new methodology to physically displace nonspecifically bound molecules from solid surfaces by utilizing a newly discovered ‘‘tuneable force’’, induced by an applied alternating electric field, which occurs within few nanometers of an electrode surface. This methodology thus offers a unique ability to shear-off loosely bound molecules from the solid/liquid interface. Via this approach, we achieved a 5-fold reduction in nonspecific adsorption of non-target protein molecules and a 1000-fold enhancement for the specific capture of HER2 protein in human serum.
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID CG-08-07
CG-12-07
Institutional Status UQ
Additional Notes Published 16 January 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
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Created: Wed, 01 Jan 2014, 06:30:32 EST by Ramanathan Pudhukode Vaidyanathan on behalf of Aust Institute for Bioengineering & Nanotechnology