Unlocking the puzzling biology of the Black Périgord truffle Tuber melanosporum

Islam, Mohammad Tawhidul, Mohamedali, Abidali, Garg, Gagan, Khan, Javed Mohammed, Gorse, Alain-Dominique, Parsons, Jeremy, Marshall, Peter, Ranganathan, Shoba and Baker, Mark S. (2013) Unlocking the puzzling biology of the Black Périgord truffle Tuber melanosporum. Journal of Proteome Research, 12 12: 5349-5356. doi:10.1021/pr400650c


Author Islam, Mohammad Tawhidul
Mohamedali, Abidali
Garg, Gagan
Khan, Javed Mohammed
Gorse, Alain-Dominique
Parsons, Jeremy
Marshall, Peter
Ranganathan, Shoba
Baker, Mark S.
Title Unlocking the puzzling biology of the Black Périgord truffle Tuber melanosporum
Formatted title
Unlocking the puzzling biology of the Black Périgord truffle Tuber melanosporum
Journal name Journal of Proteome Research   Check publisher's open access policy
ISSN 1535-3893
1535-3907
Publication date 2013-12-01
Year available 2013
Sub-type Article (original research)
DOI 10.1021/pr400650c
Open Access Status Not Open Access
Volume 12
Issue 12
Start page 5349
End page 5356
Total pages 8
Place of publication Washington, DC United States
Publisher American Chemical Society
Language eng
Formatted abstract
The black Périgord truffle (Tuber melanosporum Vittad.) is a highly prized food today, with its unique scent (i.e., perfume) and texture. Despite these attributes, it remains relatively poorly studied, lacking "omics" information to characterize its biology and biochemistry, especially changes associated with freshness and the proteins/metabolites responsible for its organoleptic properties. In this study, we have functionally annotated the truffle proteome from the 2010 T. melanosporum genome comprising 12 771 putative nonredundant proteins. Using sequential BLAST search strategies, we identified homologues for 2587 proteins with 2486 (96.0%) fungal homologues (available from http://biolinfo.org/protannotator/blacktruffle.php). A combined 1D PAGE and high-accuracy LC-MS/MS proteomic study was employed to validate the results of the functional annotation and identified 836 (6.5%) proteins, of which 47.5% (i.e., 397) were present in our bioinformatics studies. Our study, functionally annotating 6487 black Périgord truffle proteins and confirming 836 by proteomic experiments, is by far the most comprehensive study to date contributing significantly to the scientific community. This study has resulted in the functional characterization of novel proteins to increase our biological understanding of this organism and to uncover potential biomarkers of authenticity, freshness, and perfume maturation.
Keyword Black truffle
Functional annotation
Fungal proteomics
Organoleptic
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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