Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney

Takasato, M., Er, P. X., Becroft, M., Vanslambrouck, J. M., Stanley, E. G., Elefanty, A. G. and Little, M. H. (2014) Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney. Nature Cell Biology, 16 1: 118-126. doi:10.1038/ncb2894


Author Takasato, M.
Er, P. X.
Becroft, M.
Vanslambrouck, J. M.
Stanley, E. G.
Elefanty, A. G.
Little, M. H.
Title Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney
Journal name Nature Cell Biology   Check publisher's open access policy
ISSN 1465-7392
1476-4679
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1038/ncb2894
Open Access Status Not yet assessed
Volume 16
Issue 1
Start page 118
End page 126
Total pages 18
Place of publication London, United Kingdom
Publisher Nature Publishing
Language eng
Abstract With the prevalence of end-stage renal disease rising 8% per annum globally, there is an urgent need for renal regenerative strategies. The kidney is a mesodermal organ that differentiates from the intermediate mesoderm (IM) through the formation of a ureteric bud (UB) and the interaction between this bud and the adjacent IM-derived metanephric mesenchyme (MM). The nephrons arise from a nephron progenitor population derived from the MM (ref. ). The IM itself is derived from the posterior primitive streak. Although the developmental origin of the kidney is well understood, nephron formation in the human kidney is completed before birth. Hence, there is no postnatal stem cell able to replace lost nephrons. In this study, we have successfully directed the differentiation of human embryonic stem cells (hESCs) through posterior primitive streak and IM under fully chemically defined monolayer culture conditions using growth factors used during normal embryogenesis. This differentiation protocol results in the synchronous induction of UB and MM that forms a self-organizing structure, including nephron formation, in vitro. Such hESC-derived components show broad renal potential ex vivo, illustrating the potential for pluripotent-stem-cell-based renal regeneration.
Keyword Cell Biology
Cell Biology
CELL BIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID SRI110001002
APP1041277
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 18 Dec 2013, 00:01:41 EST by Susan Allen on behalf of Institute for Molecular Bioscience