A Nodal-independent and tissue-intrinsic mechanism controls heart-looping chirality

Noel, Emily S., Verhoeven, Manon, Lagendijk, Anne Karine, Tessadori, Federico, Smith, Kelly, Choorapoikayil, Suma, Den Hertog, Jeroen and Bakkers, Jeroen (2013) A Nodal-independent and tissue-intrinsic mechanism controls heart-looping chirality. Nature Communications, 4 . doi:10.1038/ncomms3754

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Author Noel, Emily S.
Verhoeven, Manon
Lagendijk, Anne Karine
Tessadori, Federico
Smith, Kelly
Choorapoikayil, Suma
Den Hertog, Jeroen
Bakkers, Jeroen
Title A Nodal-independent and tissue-intrinsic mechanism controls heart-looping chirality
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1038/ncomms3754
Open Access Status File (Publisher version)
Volume 4
Total pages 9
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Breaking left-right symmetry in bilateria is a major event during embryo development that is required for asymmetric organ position, directional organ looping and lateralized organ function in the adult. Asymmetric expression of Nodal-related genes is hypothesized to be the driving force behind regulation of organ laterality. Here we identify a Nodal-independent mechanism that drives asymmetric heart looping in zebrafish embryos. In a unique mutant defective for the Nodal-related southpaw gene, preferential dextral looping in the heart is maintained, whereas gut and brain asymmetries are randomized. As genetic and pharmacological inhibition of Nodal signalling does not abolish heart asymmetry, a yet undiscovered mechanism controls heart chirality. This mechanism is tissue intrinsic, as explanted hearts maintain ex vivo retain chiral looping behaviour and require actin polymerization and myosin II activity. We find that Nodal signalling regulates actin gene expression, supporting a model in which Nodal signalling amplifies this tissue-intrinsic mechanism of heart looping.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 864.08.009
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 32 times in Scopus Article | Citations
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Created: Tue, 17 Dec 2013, 21:58:51 EST by Susan Allen on behalf of Institute for Molecular Bioscience