Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility

Kaewmala, K., Uddin, M. J., Cinar, M. U., Grosse-Brinkhaus, C., Jonas, E., Tesfaye, D., Phatsara, C., Tholen, E., Looft, C. and Schellander, K. (2012) Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility. Reproduction in Domestic Animals, 47 2: 213-223. doi:10.1111/j.1439-0531.2011.01831.x


Author Kaewmala, K.
Uddin, M. J.
Cinar, M. U.
Grosse-Brinkhaus, C.
Jonas, E.
Tesfaye, D.
Phatsara, C.
Tholen, E.
Looft, C.
Schellander, K.
Title Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
Journal name Reproduction in Domestic Animals   Check publisher's open access policy
ISSN 0936-6768
1439-0531
Publication date 2012-04-01
Year available 2012
Sub-type Article (original research)
DOI 10.1111/j.1439-0531.2011.01831.x
Volume 47
Issue 2
Start page 213
End page 223
Total pages 11
Place of publication Berlin, Germany
Publisher Wiley-Blackwell Verlag GmbH
Collection year 2013
Language eng
Formatted abstract
 Contents: Phospholipase C zeta (PLCz) and cyclooxygenase isoenzyme type 2 (COX-2) are important in spermatogenesis, but their effect has not yet confirmed in pigs. Therefore, this study was aimed to analyse their association with sperm quality and fertility and to identify the mRNA and protein expression in boars reproductive tissues. DNA samples from 231 Pietrain (PI) and 109 Pietrain×Hampshire (PIHA) pigs with records of sperm quality [sperm concentration (SCON), motility, semen volume, plasma droplet and abnormal spermatozoa rate] and fertility (non-return rate and number of piglet born alive) traits were available. A SNP in non-coding region of PLCz g.158 A>C was associated with SCON (p<0.05) in PIHA population while the polymorphism of COX-2 g.68G>A in 3' UTR was not associated with any traits. For mRNA and protein expression study, a total of six boars were divided into two groups with G-I and G-II, where G-I was characterized for relatively better sperm quality. Both genes expressed higher in reproductive tissues compared with non-reproductive tissues. Phospholipase C zeta mRNA expressed higher in testis (p<0.01), all parts of epididymis and spermatozoa from G-I, while COX-2 expressed higher in testis (p<0.05), head and body of epididymis (p<0.01), and spermatozoa from G-II boar. Both proteins were localized in Leydig cells and spermatozoa. These results might shed light on roles of these genes in spermatogenesis as candidate for boar sperm quality and fertility, but still the lack of association across populations should be considered.
Keyword Quantitative Trait Loci
Oscillation-Inducing Activity
Resource Population
Genome Scan
Reproductive Traits
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 14 Dec 2013, 04:53:22 EST by System User on behalf of School of Veterinary Science