Expression patterns of porcine Toll-like receptors family set of genes (TLR1-10) in gut-associated lymphoid tissues alter with age

Uddin, Muhammad Jasim, Kaewmala, Kanokwan, Tesfaye, Dawit, Tholen, Ernst, Looft, Christian, Hoelker, Michael, Schellander, Karl and Cinar, Mehmet Ulas (2013) Expression patterns of porcine Toll-like receptors family set of genes (TLR1-10) in gut-associated lymphoid tissues alter with age. Research in Veterinary Science, 95 1: 92-102. doi:10.1016/j.rvsc.2013.01.027


Author Uddin, Muhammad Jasim
Kaewmala, Kanokwan
Tesfaye, Dawit
Tholen, Ernst
Looft, Christian
Hoelker, Michael
Schellander, Karl
Cinar, Mehmet Ulas
Title Expression patterns of porcine Toll-like receptors family set of genes (TLR1-10) in gut-associated lymphoid tissues alter with age
Journal name Research in Veterinary Science   Check publisher's open access policy
ISSN 0034-5288
1532-2661
Publication date 2013-08-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.rvsc.2013.01.027
Volume 95
Issue 1
Start page 92
End page 102
Total pages 11
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
The aim was to study the expression pattern of the porcine TLR family (TLR1-10) genes in gut-associated lymphoid tissues (GALT) of varying ages. A total of nine clinically healthy pigs of three ages group (1 day, 2 months and 5 months old) were selected for this experiment (three pigs in each group). Tissues from intestinal mucosa in stomach, duodenum, jejunum and ileum and mesenteric lymph node (MLN) were used. mRNA expression of TLRs (1–10) was detectable in all tissues and TLR3 showed the highest mRNA abundance among TLRs. TLR3 expression in stomach, and TLR1 and TLR6 expression in MLN were higher in adult than newborn pigs. The western blot results of TLR2, 3 and 9 in some cases, did not coincide with the mRNA expression results. The protein localization of TLR2, 3 and 9 showed that TLR expressing cells were abundant in the lamina propria, Peyer’s patches in intestine, and around and within the lymphoid follicles in the MLN. This expressions study sheds the first light on the expression patterns of all TLR genes in GALT at different ages of pigs.
Keyword Newborn
Young
Adult
TLRs
GALT
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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