Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide

Uddin, Muhammad Jasim, Nuro-Gyina, Patrick Kwadwo, Islam, Mohammad Ariful, Tesfaye, Dawit, Tholen, Ernst, Looft, Christian, Schellander, Karl and Cinar, Mehmet Ulas (2012) Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide. Veterinary Immunology and Immunopathology, 147 3-4: 211-222. doi:10.1016/j.vetimm.2012.04.020


Author Uddin, Muhammad Jasim
Nuro-Gyina, Patrick Kwadwo
Islam, Mohammad Ariful
Tesfaye, Dawit
Tholen, Ernst
Looft, Christian
Schellander, Karl
Cinar, Mehmet Ulas
Title Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide
Journal name Veterinary Immunology and Immunopathology   Check publisher's open access policy
ISSN 0165-2427
1873-2534
Publication date 2012-06-01
Sub-type Article (original research)
DOI 10.1016/j.vetimm.2012.04.020
Volume 147
Issue 3-4
Start page 211
End page 222
Total pages 12
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract The Toll-like receptor (TLR)4 is critical for the recognition of Gram-negative bacterial lipopolysaccharide (LPS) but in porcine peripheral blood mononuclear cells (PBMCs) it may cooperate with other TLRs and lead to the production of inflammatory cytokines. Therefore, we analyzed TLR1-10 mRNA expression in porcine PBMCs stimulated with LPS over time (1-48. h) by using quantitative real-time PCR and cytokine proteins level by ELISA in culture supernatant. TLR1-10 mRNA was detectable in porcine PBMCs. When compared with the control (non-stimulated), TLR1 mRNA were increased (p< 0.05) at 3. h after challenge with 1 μg/ml LPS, whereas TLR1 and TLR2 mRNA were increased (p< 0.01) at 6. h after challenge with 10 μg/ml LPS. TLR4 increased (p< 0.001) at 3. h after challenge with LPS and remained constant. TLR5 and TLR6 mRNA increased (p< 0.05) at 9. h and 1. h after of LPS stimulation, respectively. The mRNA of CD14 and MD2 were increased (p< 0.001) at 1. h after LPS stimulation. Additionally, at most of the time analyzed, the mRNA expression increased with the dose of LPS. The LPS concentration had influence (p< 0.05) on all the TLRs expression except TLR10; whereas time had effect (p< 0.05) on all TLRs expression except TLR2, 3, 6 and 10. When compared to the control, the cytokines IL1b, IL8 and TNFα proteins were increased (p< 0.001) immediately at 1. h after LPS stimulation and remained constant till 48. h. IL12b was increased (p< 0.001) 12. h after challenge with 10 μg/ml of LPS. Although IL8 level was the highest, the higher (p< 0.05) expression of all these inflammatory cytokines indicate that upon interacting with TLRs, LPS exerted inflammatory response in PBMCs through the production of Th1 type cytokines. The production of cytokines was influenced (p< 0.001) by both the dose of LPS and the stimulation time. Hence, the porcine PBMCs are likely able to express all members of TLRs.
Keyword TLRs
PBMCs
Cytokines
LPS
Innate immune response
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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