Mapping quantitative trait loci for innate immune response in the pig

Uddin, M. J., Cinar, M. U., Grosse-Brinkhaus, C., Tesfaye, D., Tholen, E., Juengst, H., Looft, C., Wimmers, K., Phatsara, C. and Schellander, K. (2011) Mapping quantitative trait loci for innate immune response in the pig. International Journal of Immunogenetics, 38 2: 121-131. doi:10.1111/j.1744-313X.2010.00985.x


Author Uddin, M. J.
Cinar, M. U.
Grosse-Brinkhaus, C.
Tesfaye, D.
Tholen, E.
Juengst, H.
Looft, C.
Wimmers, K.
Phatsara, C.
Schellander, K.
Title Mapping quantitative trait loci for innate immune response in the pig
Journal name International Journal of Immunogenetics   Check publisher's open access policy
ISSN 1744-3121
1744-313X
Publication date 2011-04-01
Year available 2011
Sub-type Article (original research)
DOI 10.1111/j.1744-313X.2010.00985.x
Volume 38
Issue 2
Start page 121
End page 131
Total pages 11
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Language eng
Formatted abstract
 The aim of the present study was to detect quantitative trait loci (QTL) for the serum levels of cytokines and Toll-like receptors as traits related to innate immunity in pig. For this purpose, serum concentration of interleukin 2 (IL2), interleukin 10 (IL10), interferon-gamma (IFNG), Toll-like receptor 2 (TLR2) and Toll-like receptor 9 (TLR9) were measured in blood samples obtained from F 2 piglets (n=334) of a Duroc×Piétrain resource population (DUPI) after Mycoplasma hypopneumoniae (Mh), tetanus toxoid (TT) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) vaccination at 6, 9 and 15weeks of age. Animals were genotyped at 82 genetic markers covering all autosomes. QTL analysis was performed under the line cross F 2 model using QTL Express and 33 single QTL were detected on almost all porcine autosomes. Among the single QTL, eight, twelve and thirteen QTL were identified for innate immune traits in response to Mh, TT and PRRSV vaccine, respectively. Besides single QTL, six QTL were identified by a two-QTL model, of which two for TLR9-TT were in coupling phase and one for IL10-PRRSV was in repulsion phase. All QTL were significant at 5% chromosome-wide level including one and seven at 5% genome- and 1% chromosome-wide level significance. All innate immune traits are influenced by multiple chromosomal regions implying multiple gene action. Some of the identified QTL coincided with previously reported QTL for immune response and disease resistance, and the newly identified QTL are potentially involved in the immune function. The immune traits were also influenced by environmental factors like year of birth, age, parity and litter size. The results of this work shed new light on the genetic background of innate immune response and these findings will be helpful to identify candidate genes in these QTL regions related to immune competence and disease resistance in pigs
Keyword Pietrain Resource Population
Combined Line Cross
Rapid Communication
Signaling Pathways
Interferon gamma
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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