Waveguide evanescent field scattering microscopy: bacterial biofilms and their sterilization response via UV irradiation

Nahar, Qamrun, Fleißner, Frederik, Shuster, Jeremiah, Morawitz, Michael, Halfpap, Christopher, Stefan, Mihaela, Langbein, Uwe, Southam, Gordon and Mittler, Silvia (2014) Waveguide evanescent field scattering microscopy: bacterial biofilms and their sterilization response via UV irradiation. Journal of Biophotonics, 7 7: 542-551. doi:10.1002/jbio.201300135

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Author Nahar, Qamrun
Fleißner, Frederik
Shuster, Jeremiah
Morawitz, Michael
Halfpap, Christopher
Stefan, Mihaela
Langbein, Uwe
Southam, Gordon
Mittler, Silvia
Title Waveguide evanescent field scattering microscopy: bacterial biofilms and their sterilization response via UV irradiation
Journal name Journal of Biophotonics   Check publisher's open access policy
ISSN 1864-063X
1864-0648
Publication date 2014-07-01
Year available 2013
Sub-type Article (original research)
DOI 10.1002/jbio.201300135
Open Access Status
Volume 7
Issue 7
Start page 542
End page 551
Total pages 10
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag
Language eng
Abstract Waveguide Evanescent Field Scattering (WEFS) microscopy is introduced as a new and simple tool for label-free, high contrast imaging of bacteria and bacteria sensors. Bacterial microcolonies and single bacteria were discriminated both by their bright field images and by their evanescent scattering intensity. By comparing bright field images with WEFS images, the proportion of planktonic: sessile (i.e., "floating": attached) bacteria were measured. Bacteria were irradiated with UV light, which limited their biofilm forming capability. A quantitative decrease in attachment of individual, sessile bacteria and in attached, microcolony occupied areas was easily determined within the apparent biofilms with increasing UV dose. WEFS microscopy is an ideal tool for providing rapid quantitative data on biofilm formation.
Keyword Biofilm
Evanescent field
Microscopy
Scattering
UV sterilization
Waveguide
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 18 October 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Earth Sciences Papers
 
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Created: Sat, 14 Dec 2013, 00:43:01 EST by Ashleigh Paroz on behalf of School of Earth Sciences