Inactivation kinetics of anaerobic wastewater biofilms by free nitrous acid

Jiang, Guangming and Yuan, Zhiguo (2013) Inactivation kinetics of anaerobic wastewater biofilms by free nitrous acid. Applied Microbiology and Biotechnology, 98 3: 1367-1376. doi:10.1007/s00253-013-5031-6

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Author Jiang, Guangming
Yuan, Zhiguo
Title Inactivation kinetics of anaerobic wastewater biofilms by free nitrous acid
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
Publication date 2013-06-18
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s00253-013-5031-6
Volume 98
Issue 3
Start page 1367
End page 1376
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Recent studies have shown that free nitrous acid (FNA) is biocidal to a broad range of microorganisms. Microorganisms residing in anaerobic sewer biofilms were found to be inactivated after a short (6–24 h) exposure to FNA. In this study, we investigate the inactivation kinetics of anaerobic sewer biofilms grown in real wastewater. Microbial viability of biofilms was determined using LIVE/DEAD staining. A two-fraction kinetic model was developed to simulate the inactivation of mixed culture in biofilms. The kinetic parameters were estimated by using Bayesian statistics. Model simulation found that a fraction (85 %) of the biofilm community was highly sensitive to FNA with a high inactivation rate, and a fraction (15 %) was tolerant to FNA and persisted after FNA treatment. This different susceptibility to FNA treatment was likely due to the diverse microbial community and biofilm protection. The fact that nearly 85 % microbes were inactivated confirmed that FNA is a strong biocide to mixed-culture biofilms. It was found that the inactivation rate constant was not affected by pH levels. The kinetic model was successfully used to optimize FNA dosage for sulfide control in sewer biofilms. Also, results suggest that a high FNA concentration is preferred than long exposure time to reduce the total chemical consumption.
Keyword Inactivation kinetics
Wastewater biofilm
Free nitrous acid
Bayesian statistics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 18 June 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
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Created: Wed, 11 Dec 2013, 23:39:15 EST by Mr Guangming Jiang on behalf of Advanced Water Management Centre