Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease

Hoier, Birgitte, Walker, Meegan, Passos, Madla, Walker, Philip J., Green, Anita, Bangsbo, Jens, Askew, Christopher D. and Hellsten, Ylva (2013) Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease. Journal of Applied Physiology, 115 12: 1777-1787. doi:10.1152/japplphysiol.00979.2013


Author Hoier, Birgitte
Walker, Meegan
Passos, Madla
Walker, Philip J.
Green, Anita
Bangsbo, Jens
Askew, Christopher D.
Hellsten, Ylva
Title Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease
Journal name Journal of Applied Physiology   Check publisher's open access policy
ISSN 0161-7567
1522-1601
Publication date 2013-12-01
Year available 2013
Sub-type Article (original research)
DOI 10.1152/japplphysiol.00979.2013
Volume 115
Issue 12
Start page 1777
End page 1787
Total pages 11
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Language eng
Abstract Peripheral arterial disease (PAD) is caused by atherosclerosis and is associated with microcirculatory impairments in skeletal muscle. The present study evaluated the angiogenic response to exercise and passive movement in skeletal muscle of PAD patients compared with healthy control subjects. Twenty-one PAD patients and 17 aged control subjects were randomly assigned to either a passive movement or an active exercise study. Interstitial fluid microdialysate and tissue samples were obtained from the thigh skeletal muscle. Muscle dialysate vascular endothelial growth factor (VEGF) levels were modestly increased in response to either passive movement or active exercise in both subject groups. The basal muscle dialysate level of the angiostatic factor thrombospondin-1 protein was markedly higher (P < 0.05) in PAD patients compared with the control subjects, whereas soluble VEGF receptor-1 dialysate levels were similar in the two groups. The basal VEGF protein content in the muscle tissue samples was 27% lower (P < 0.05) in the PAD patients compared with the control subjects. Analysis of mRNA expression for a range of angiogenic and angiostatic factors revealed a modest change with active exercise and passive movement in both groups, except for an increase (P < 0.05) in the ratio of angiopoietin-2 to angiopoietin-1 mRNA in the PAD group with both interventions. PAD patients and aged individuals showed a similar limited angiogenic response to active exercise and passive movement. The limited increase in muscle extracellular VEGF combined with an elevated basal level of thrombospondin-1 in muscle extracellular fluid of PAD patients may restrict capillary growth in these patients.
Keyword Capillary
Intermittent claudication
Microdialysis
Skeletal muscle
Vascular endothelial growth factor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 11 Dec 2013, 21:48:11 EST by Roheen Gill on behalf of UQ Centre for Clinical Research