Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae

Velkov, Tony, Deris, Zakuan Z., Huang, Johnny X., Azad, Mohammad A. K., Butler, Mark, Sivanesan, Sivashangarie, Kiminskas, Lisa M., Dong, Yao-Da, Boyd, Ben, Baker, Mark A., Cooper, Matthew A., Nation, Roger L. and Li, Jian (2013) Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae. Innate Immunity, 20 4: 350-363. doi:10.1177/1753425913493337

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Author Velkov, Tony
Deris, Zakuan Z.
Huang, Johnny X.
Azad, Mohammad A. K.
Butler, Mark
Sivanesan, Sivashangarie
Kiminskas, Lisa M.
Dong, Yao-Da
Boyd, Ben
Baker, Mark A.
Cooper, Matthew A.
Nation, Roger L.
Li, Jian
Title Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae
Formatted title
Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae
Journal name Innate Immunity   Check publisher's open access policy
ISSN 1753-4259
1753-4267
Publication date 2013-07-25
Year available 2013
Sub-type Article (original research)
DOI 10.1177/1753425913493337
Volume 20
Issue 4
Start page 350
End page 363
Total pages 14
Place of publication London, United Kingdom
Publisher Sage Publications
Language eng
Formatted abstract
This study examines the interaction of polymyxin B and colistin with the surface and outer membrane components of a susceptible and resistant strain of Klebsiella pneumoniae. The interaction between polymyxins and bacterial membrane and isolated LPS from paired wild type and polymyxin-resistant strains of K. pneumoniae were examined with N-phenyl-1-naphthylamine (NPN) uptake, fluorometric binding and thermal shift assays, lysozyme and deoxycholate sensitivity assays, and by 1H NMR. LPS from the polymyxin-resistant strain displayed a reduced binding affinity for polymyxins B and colistin in comparison with the wild type LPS. The outer membrane NPN permeability of the resistant strain was greater compared with the susceptible strain. Polymyxin exposure enhanced the permeability of the outer membrane of the wild type strain to lysozyme and deoxycholate, whereas polymyxin concentrations up to 32 mg/ml failed to permeabilize the outer membrane of the resistant strain. Zeta potential measurements revealed that mid-logarithmic phase wild type cells exhibited a greater negative charge than the mid-logarithmic phase-resistant cells. Taken together, our findings suggest that the resistant derivative of K. pneumoniae can block the electrostatically driven first stage of polymyxin action, which thereby renders the hydrophobically driven second tier of polymyxin action on the outer membrane inconsequential.
Keyword Polymyxin
Colistin
Klebsiella pneumoniae
Lipopolysaccharide
Surface
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 25 July 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
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Created: Wed, 11 Dec 2013, 01:37:04 EST by Susan Allen on behalf of Institute for Molecular Bioscience