Correlation of adrenomedullin gene expression in peripheral blood leukocytes with severity of ischemic stroke

Liu, Jia, Yan, Jun, Greer, Judith M., Read, Stephen J., Henderson, Robert D., Rose, Stephen E., Coulthard, Alan and McCombe, Pamela A. (2014) Correlation of adrenomedullin gene expression in peripheral blood leukocytes with severity of ischemic stroke. International Journal of Neuroscience, 124 4: 271-280. doi:10.3109/00207454.2013.837462

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Author Liu, Jia
Yan, Jun
Greer, Judith M.
Read, Stephen J.
Henderson, Robert D.
Rose, Stephen E.
Coulthard, Alan
McCombe, Pamela A.
Title Correlation of adrenomedullin gene expression in peripheral blood leukocytes with severity of ischemic stroke
Journal name International Journal of Neuroscience   Check publisher's open access policy
ISSN 0020-7454
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.3109/00207454.2013.837462
Open Access Status File (Author Post-print)
Volume 124
Issue 4
Start page 271
End page 280
Total pages 10
Place of publication London, United Kingdom
Publisher Informa Healthcare
Language eng
Formatted abstract
Human adrenomedullin (ADM), a 52-amino acid peptide, belongs to the calcitonin/calcitonin gene-related peptide (CGRP)/amylin peptide family. ADM acts as a multifunctional regulatory peptide and is upregulated in response to hypoxia. Previous microarray studies have found increased ADM gene (ADM) expression in peripheral blood cells of patients with stroke, however, it is unknown if an increased ADM level is correlated with severity of human ischemic stroke. This study investigated ADM expression in peripheral blood leukocytes (PBL) of healthy controls and subjects at day 1, week 1 and week 3 postacute ischemic stroke using rtPCR methodology. We found that ADM expression was significantly upregulated on the first day of stroke compared to the healthy subjects and the disease controls; the levels remained elevated for up to week 3. Further, ADM expression at day 1 was correlated with stroke severity measured by the National Institute of Healthy Stroke Scale (NIHSS), the modified Barthel Index (mBI) and the modified Rankin Scale (mRS). This could indicate that ADM expression level is related to the severity of tissue damage. We suggest that increased ADM expression in PBL after acute ischemic stroke is most likely to indicate that these cells have been subjected to hypoxia and that the magnitude of expression is likely to be related to the volume of hypoxic tissue. Hypoxia can affect lymphocytes function and could affect the immune response to stroke. The correlation of ADM expression level with the measures of stroke severity implicates ADM –a potential blood bio-marker in studies of ischemic stroke.
Keyword Gene expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Wed, 11 Dec 2013, 00:03:35 EST by Roheen Gill on behalf of UQ Centre for Clinical Research