Drivers of microbial community composition in mesophilic and thermophilic temperature-phased anaerobic digestion pre-treatment reactors

Pervin, Hasina M., Dennis, Paul G., Lim, Hui J., Tyson, Gene W., Batstone, Damien J. and Bond, Philip L. (2013) Drivers of microbial community composition in mesophilic and thermophilic temperature-phased anaerobic digestion pre-treatment reactors. Water Research, 47 19: 7098-7108. doi:10.1016/j.watres.2013.07.053


Author Pervin, Hasina M.
Dennis, Paul G.
Lim, Hui J.
Tyson, Gene W.
Batstone, Damien J.
Bond, Philip L.
Title Drivers of microbial community composition in mesophilic and thermophilic temperature-phased anaerobic digestion pre-treatment reactors
Journal name Water Research   Check publisher's open access policy
ISSN 0043-1354
1879-2448
Publication date 2013-12-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.watres.2013.07.053
Volume 47
Issue 19
Start page 7098
End page 7108
Total pages 11
Place of publication London, United Kingdom
Publisher I W A Publishing
Language eng
Subject 2312 Water Science and Technology
2311 Waste Management and Disposal
2310 Pollution
2302 Ecological Modelling
Abstract Temperature-phased anaerobic digestion (TPAD) is an emerging technology that facilitates improved performance and pathogen destruction in anaerobic sewage sludge digestion by optimising conditions for 1) hydrolytic and acidogenic organisms in a first-stage/pre-treatment reactor and then 2) methogenic populations in a second stage reactor. Pre-treatment reactors are typically operated at 55-65°C and as such select for thermophilic bacterial communities. However, details of key microbial populations in hydrolytic communities and links to functionality are very limited. In this study, experimental thermophilic pre-treatment (TP) and control mesophilic pre-treatment (MP) reactors were operated as first-stages of TPAD systems treating activated sludge for 340 days. The TP system was operated sequentially at 50, 60 and 65°C, while the MP rector was held at 35°C for the entire period. The composition of microbial communities associated with the MP and TP pre-treatment reactors was characterised weekly using terminal-restriction fragment length polymorphism (T-RFLP) supported by clone library sequencing of 16S rRNA gene amplicons. The outcomes of this approach were confirmed using 454 pyrosequencing of gene amplicons and fluorescence in-situ hybridisation (FISH). TP associated bacterial communities were dominated by populations affiliated to the Firmicutes, Thermotogae, Proteobacteria and Chloroflexi. In particular there was a progression from Thermotogae to Lutispora and Coprothermobacter and diversity decreased as temperature and hydrolysis performance increased. While change in the composition of TP associated bacterial communities was attributable to temperature, that of MP associated bacterial communities was related to the composition of the incoming feed. This study determined processes driving the dynamics of key microbial populations that are correlated with an enhanced hydrolytic functionality of the TPAD system.
Keyword Hydrolysis fermentation
Molecular microbial ecology
Temperature phased anaerobic digestion
Thermophilic pre treatment
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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