Genetic diversity of Chlamydia pecorum strains in wild koala locations across Australia and the implications for a recombinant C. pecorum major outer membrane protein based vaccine

Kollipara, Avinash, Polkinghorne, Adam, Wan, Charles, Kanyoka, Pride, Hanger, Jon, Loader, Joanne, Callaghan, John, Bell, Alicia, Ellis, William, Fitzgibbon, Sean, Melzer, Alistar, Beagley, Kenneth and Timms, Peter (2013) Genetic diversity of Chlamydia pecorum strains in wild koala locations across Australia and the implications for a recombinant C. pecorum major outer membrane protein based vaccine. Veterinary Microbiology, 167 3-4: 513-522. doi:10.1016/j.vetmic.2013.08.009


Author Kollipara, Avinash
Polkinghorne, Adam
Wan, Charles
Kanyoka, Pride
Hanger, Jon
Loader, Joanne
Callaghan, John
Bell, Alicia
Ellis, William
Fitzgibbon, Sean
Melzer, Alistar
Beagley, Kenneth
Timms, Peter
Title Genetic diversity of Chlamydia pecorum strains in wild koala locations across Australia and the implications for a recombinant C. pecorum major outer membrane protein based vaccine
Formatted title
Genetic diversity of Chlamydia pecorum strains in wild koala locations across Australia and the implications for a recombinant C. pecorum major outer membrane protein based vaccine
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 2013-12-27
Sub-type Article (original research)
DOI 10.1016/j.vetmic.2013.08.009
Volume 167
Issue 3-4
Start page 513
End page 522
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 2404 Microbiology
3400 Veterinary
Formatted abstract
The long term survival of the koala ( Phascolarctos cinereus) is at risk due to a range of threatening processes. A major contributing factor is disease caused by infection with Chlamydia pecorum, which has been detected in most mainland koala populations and is associated with ocular and genital tract infections. A critical aspect for the development of vaccines against koala chlamydial infections is a thorough understanding of the prevalence and strain diversity of C. pecorum infections across wild populations. In this study, we describe the largest survey (403 koalas from eight wild populations and three wildlife hospitals) examining the diversity of C. pecorum infections. 181 of the 403 koalas tested (45%) positive for C. pecorum by species-specific quantitative PCR with infection rates ranging from 20% to 61% in the eight wild populations sampled. The ompA gene, which encodes the chlamydial major outer membrane protein (MOMP), has been the major target of several chlamydial vaccines. Based on our analysis of the diversity of MOMP amino types in the infected koalas, we conclude that, (a) there exists significant diversity of C. pecorum strains in koalas, with 10 distinct, full length C. pecorum MOMP amino types identified in the 11 koala locations sampled, (b) despite this diversity, there are predicted T and B cell epitopes in both conserved as well as variable domains of MOMP which suggest cross-amino type immune responses, and (c) a recombinant MOMP-based vaccine consisting of MOMP "F" could potentially induce heterotypic protection against a range of C. pecorum strains.
Keyword Amino type
Chlamydia pecorum
Diversity
Koala
MOMP
ompA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Sustainable Minerals Institute Publications
 
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