Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

Daumann, Lena J., Larrabee, James A., Ollis, David, Schenk, Gerhard and Gahan, Lawrence R. (2014) Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation. Journal of Inorganic Biochemistry, 131 1-7. doi:10.1016/j.jinorgbio.2013.10.007

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Author Daumann, Lena J.
Larrabee, James A.
Ollis, David
Schenk, Gerhard
Gahan, Lawrence R.
Title Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation
Journal name Journal of Inorganic Biochemistry   Check publisher's open access policy
ISSN 0162-0134
1873-3344
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jinorgbio.2013.10.007
Open Access Status Not yet assessed
Volume 131
Start page 1
End page 7
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Abstract Annually thousands of people die or suffer from organophosphate (pesticide) poisoning. In order to remove these toxic compounds from the environment, the use of enzymes as bioremediators has been proposed. We report here a Ser127Ala mutant based on the enzyme glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes. The mutant, with improved metal binding abilities, has been immobilized using glutaraldehyde on PAMAM dendrimer-modified magnetite nanoparticles. The immobilized system was characterized using elemental analysis as well as infrared, transmission electron and X-ray photoelectron spectroscopies. The amount of GpdQ that was immobilized with the optimized procedure was 1.488 nmol per g MNP. A kinetic assay has been designed to evaluate the activity of the system towards organophosphoester substrates. The specific activity towards BPNPP directly after immobilization was 3.55 μmol mg- 1 min- 1, after one week 3.39 μmol mg- 1 min- 1 and after 120 days 3.36 μmol mg- 1 min - 1, demonstrating that the immobilized enzyme was active for multiple cycles and could be stored on the nanoparticles for a prolonged period.
Keyword Bioremediation
Magnetite nanoparticles
Metalloenzyme
Organophosphate pesticide
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP0986613
CHE 0848433
Institutional Status UQ
Additional Notes Available online: 16 October 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
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