Chemical inhibition of acetyl coenzyme A carboxylase as a strategy to increase polyhydroxybutyrate yields in transgenic sugarcane

Petrasovits, Lars A., McQualter, Richard B., Gebbie, Leigh K., Blackman, Deborah M., Nielsen, Lars K. and Brumbley, Stevens M. (2013) Chemical inhibition of acetyl coenzyme A carboxylase as a strategy to increase polyhydroxybutyrate yields in transgenic sugarcane. Plant Biotechnology Journal, 11 9: 1146-1151. doi:10.1111/pbi.12109


Author Petrasovits, Lars A.
McQualter, Richard B.
Gebbie, Leigh K.
Blackman, Deborah M.
Nielsen, Lars K.
Brumbley, Stevens M.
Title Chemical inhibition of acetyl coenzyme A carboxylase as a strategy to increase polyhydroxybutyrate yields in transgenic sugarcane
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7644
1467-7652
Publication date 2013-12-01
Year available 2013
Sub-type Article (original research)
DOI 10.1111/pbi.12109
Open Access Status DOI
Volume 11
Issue 9
Start page 1146
End page 1151
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject 1110 Nursing
1305 Biotechnology
1102 Cardiovascular Medicine and Haematology
Formatted abstract
Polyhydroxybutyrate (PHB) is a naturally occurring bacterial polymer that can be used as a biodegradable replacement for some petrochemical-derived plastics. Polyhydroxybutyrate is produced commercially by fermentation, but to reduce production costs, efforts are underway to produce it in engineered plants, including sugarcane. However, PHB levels in this high-biomass crop are not yet commercially viable. Chemical ripening with herbicides is a strategy used to enhance sucrose production in sugarcane and was investigated here as a tool to increase PHB production. Class A herbicides inhibit ACCase activity and thus reduce fatty acid biosynthesis, with which PHB production competes directly for substrate. Treatment of PHB-producing transgenic sugarcane plants with 100 μm of the class A herbicide fluazifop resulted in a fourfold increase in PHB content in the leaves, which peaked ten days post-treatment. The minimum effective concentration of herbicide required to maximize PHB production was 30 μm for fluazifop and 70 μm for butroxydim when applied to saturation. Application of a range of class A herbicides from the DIM and FOP groups consistently resulted in increased PHB yields, particularly in immature leaf tissue. Butroxydim or fluazifop treatment of mature transgenic sugarcane grown under glasshouse conditions increased the total leaf biomass yield of PHB by 50%-60%. Application of an ACCase inhibitor in the form of a class A herbicide to mature sugarcane plants prior to harvest is a promising strategy for improving overall PHB yield. Further testing is required on field-grown transgenic sugarcane to more precisely determine the effectiveness of this strategy.
Keyword Polyhydroxybutyrate
Sugarcane
Biopolymer
Chemical ripener
Herbicide
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID LP100100659
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 04 Dec 2013, 20:28:52 EST by Dr Richard Mcqualter on behalf of Aust Institute for Bioengineering & Nanotechnology