The quantitative real-time polymerase chain reaction for the analysis of plant gene expression

Fitzgerald, Timothy L. and McQualter, Richard B. (2014). The quantitative real-time polymerase chain reaction for the analysis of plant gene expression. In Robert J. Henry, Agnelo Furtado and John M. Walker (Ed.), Cereal Genomics: Methods and Protocols (pp. 97-115) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-715-0


Author Fitzgerald, Timothy L.
McQualter, Richard B.
Title of chapter The quantitative real-time polymerase chain reaction for the analysis of plant gene expression
Title of book Cereal Genomics: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2014
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-62703-715-0
Open Access Status Not yet assessed
Series Methods in Molecular Biology
ISBN 9781627037143
9781627037150
ISSN 1064-3745
1940-6029
Editor Robert J. Henry
Agnelo Furtado
John M. Walker
Volume number 1099
Chapter number 9
Start page 97
End page 115
Total pages 19
Total chapters 24
Collection year 2015
Language eng
Formatted Abstract/Summary
The quantitative real-time polymerase chain reaction is used to simultaneously amplify and quantify a targeted DNA molecule. It can be used to determine exact copy number of a molecule within a sample and/or to compare the quantity of a molecule between samples. When combined with reverse transcription, it is a powerful tool for the analysis of gene expression, and it is widely used for this purpose in plant species. Here we provide an introduction to fundamental concepts relevant for the analysis of gene expression in plants using this technique and a protocol for quantification of the relative expression of a sucrose phosphate synthase gene along the maturation gradient of a sugarcane leaf.
Keyword Quantitative real-time PCR
Gene expression
RNA
cDNA
Thermocycler
Probe
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 04 Dec 2013, 20:17:38 EST by Dr Richard Mcqualter on behalf of Aust Institute for Bioengineering & Nanotechnology