Th17 and Th22 cells in psoriatic arthritis and psoriasis

Benham, Helen, Norris, Paul, Goodall, Jane, Wechalekar, Mihir D., FitzGerald, Oliver, Szentpetery, Agnes, Smith, Malcolm, Thomas, Ranjeny and Gaston, Hill (2013) Th17 and Th22 cells in psoriatic arthritis and psoriasis. Arthritis Research and Therapy, 15 5: . doi:10.1186/ar4317


Author Benham, Helen
Norris, Paul
Goodall, Jane
Wechalekar, Mihir D.
FitzGerald, Oliver
Szentpetery, Agnes
Smith, Malcolm
Thomas, Ranjeny
Gaston, Hill
Title Th17 and Th22 cells in psoriatic arthritis and psoriasis
Journal name Arthritis Research and Therapy   Check publisher's open access policy
ISSN 1478-6354
1478-6362
Publication date 2013-09-26
Year available 2013
Sub-type Article (original research)
DOI 10.1186/ar4317
Open Access Status DOI
Volume 15
Issue 5
Total pages 11
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Abstract Introduction: The aim of this study was to characterize interleukin 17 (IL-17) and interleukin 22 (IL-22) producing cells in peripheral blood (PB), skin, synovial fluid (SF) and synovial tissue (ST) in patients with psoriasis (Ps) and psoriatic arthritis (PsA).Methods: Flow cytometry was used to enumerate cells making IL-22 and IL-17, in skin and/or SF and PB from 11 patients with Ps and 12 patients with PsA; skin and PB of 15 healthy controls and SF from rheumatoid arthritis (RA) patients were used as controls. Expression of the interleukin 23 receptor (IL-23R) and chemokine receptors CCR4 and CCR6 was examined. Secretion of IL-17 and IL-22 was measured by ELISA. ST was analysed by immunohistochemical staining of IL-17 and IL-22.Results: Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were seen in PB of patients with PsA and Ps. IL-17 secretion was significantly elevated in both PsA and Ps, whilst IL-22 secretion was higher in PsA compared to Ps and healthy controls. A higher proportion of the CD4+ cells making IL-17 or IL-22 expressed IL-23R and frequencies of IL-17+, CCR6+ and CCR4+ T cells were elevated in patients with Ps and those with PsA. In patients with PsA, CCR6+ and IL-23R + T cells numbers were elevated in SF compared to PB. Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were demonstrated in Ps skin lesions. In contrast, whilst elevated frequencies of CD4+ IL-17+ cells were seen in PsA SF compared to PB, frequencies of CD4+ IL-22+ T cells were lower. Whereas IL-17 expression was equivalent in PsA, osteoarthritis (OA) and RA ST, IL-22 expression was higher in RA than either OA or PsA ST, in which IL-22 was strikingly absent.Conclusions: Elevated frequencies of IL-17 and IL-22 producing CD4+ T cells were a feature of both Ps and PsA. However their differing distribution at disease sites, including lower frequencies of IL-22+ CD4+ T cells in SF compared to skin and PB, and lack of IL-22 expression in ST suggests that Th17 and Th22 cells have common, as well as divergent roles in the pathogenesis of Ps and PsA.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
UQ Diamantina Institute Publications
 
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