Mapping of mitochondrial RNA-protein interactions by digital RNase footprinting

Liu, Ganqiang, Mercer, Timothy R., Shearwood, Anne-Marie J., Siira, Stefan J., Hibbs, Moira E., Mattick, John S., Rackham, Oliver and Filipovska, Aleksandra (2013) Mapping of mitochondrial RNA-protein interactions by digital RNase footprinting. Cell Reports, 5 3: 839-848. doi:10.1016/j.celrep.2013.09.036

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Author Liu, Ganqiang
Mercer, Timothy R.
Shearwood, Anne-Marie J.
Siira, Stefan J.
Hibbs, Moira E.
Mattick, John S.
Rackham, Oliver
Filipovska, Aleksandra
Title Mapping of mitochondrial RNA-protein interactions by digital RNase footprinting
Journal name Cell Reports   Check publisher's open access policy
ISSN 2211-1247
Publication date 2013-01-01
Sub-type Article (original research)
DOI 10.1016/j.celrep.2013.09.036
Open Access Status DOI
Volume 5
Issue 3
Start page 839
End page 848
Total pages 10
Place of publication New York, NY, United States
Publisher Elsevier
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
Abstract Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
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Created: Tue, 03 Dec 2013, 10:46:44 EST by System User on behalf of Institute for Molecular Bioscience