Intermediates in the guanine nucleotide exchange reaction of Rab8 protein catalyzed by guanine nucleotide exchange factors Rabin8 and GRAB

Guo, Zhong, Hou, Xiaomin, Goody, Roger S. and Itzen, Aymelt (2013) Intermediates in the guanine nucleotide exchange reaction of Rab8 protein catalyzed by guanine nucleotide exchange factors Rabin8 and GRAB. Journal of Biological Chemistry, 288 45: 32466-32474. doi:10.1074/jbc.M113.498329


Author Guo, Zhong
Hou, Xiaomin
Goody, Roger S.
Itzen, Aymelt
Title Intermediates in the guanine nucleotide exchange reaction of Rab8 protein catalyzed by guanine nucleotide exchange factors Rabin8 and GRAB
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 2013-11-08
Year available 2013
Sub-type Article (original research)
DOI 10.1074/jbc.M113.498329
Open Access Status DOI
Volume 288
Issue 45
Start page 32466
End page 32474
Total pages 9
Place of publication Bethesda, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
Small G-proteins of the Ras superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replacement of GDP by GTP. During nucleotide exchange, three distinct substrate·enzyme complexes occur: a ternary complex with GDP at the start of the reaction (G-protein·GEF·GDP), an intermediary nucleotide-free binary complex (G-protein·GEF), and a ternaryGTPcomplex after productive G-protein activation (G- protein·GEF·GTP). Here, we show structural snapshots of the full nucleotide exchange reaction sequence together with the G-protein substrates and products using Rabin8/GRAB (GEF) and Rab8 (G-protein) as a model system. Together with a thorough enzymatic characterization, our data provide a detailed view into the mechanism of Rabin8/GRAB-mediated nucleotide exchange.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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