Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma

Dalley, Andrew J., Pitty, Luke P., Major, Aidan G., AbdulMajeed, Ahmad A. and Farah, Camile S. (2014) Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma. Cancer Medicine, 3 2: 273-283. doi:10.1002/cam4.182


Author Dalley, Andrew J.
Pitty, Luke P.
Major, Aidan G.
AbdulMajeed, Ahmad A.
Farah, Camile S.
Title Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma
Journal name Cancer Medicine   Check publisher's open access policy
ISSN 2045-7634
Publication date 2014-04-01
Sub-type Article (original research)
DOI 10.1002/cam4.182
Open Access Status DOI
Volume 3
Issue 2
Start page 273
End page 283
Total pages 11
Place of publication Bognor Regis, West Sussex, United Kingdom
Publisher John Wiley & Sons
Language eng
Formatted abstract
Early diagnosis is vital for effective treatment of oral squamous cell carcinoma (OSCC). The optimal time for clinical intervention is prior to malignancy when patients present with oral potentially malignant lesions such as leukoplakia or erythroplakia. Transformation rates for oral dysplasia vary greatly and more rigorous methods are needed to predict the malignant potential of oral lesions. We hypothesized that the expression of two putative stem cell markers, ABCG2 and Bmi-1, would correlate with disease severity for non diseased, potentially malignant and OSCC specimens and cell lines derived from an equivalent range of tissues. We compared immunoreactive protein and relative gene expression of ABCG2 and Bmi-1 in eight cell lines derived from source tissues ranging in disease severity from normal (OKF6-TERT2) through mild and moderate/severe dysplasia (DOK, POE-9n) to OSCC (PE/CA-PJ15, SCC04, SCC25, SCC09, SCC15). We also analyzed immunoreactive protein expression of ABCG2 and Bmi-1 in 189 tissue samples with the same range of disease severity. A trend between oral lesion severity to ABCG2 and Bmi-1 immunostain intensity was observed. Flow cytometry of oral cell lines confirmed this trend and gave good correlation with RT-PCR results for ABCG2 (r = 0.919, P = 0.001; Pearson) but not Bmi-1 (r = −0.311). The results provide evidence of increased density of ABCG2 and Bmi-1-positive populations in malignant and oral potentially malignant lesions and derived cell lines, but that intragroup variability within IHC, flow cytometry, and RT-PCR results compromise the diagnostic potential of these techniques for discriminating oral dysplasia from normal tissue or OSCC.
Keyword ABCG2
Biomarkers
Bmi-1
Cell lines
Head and neck cancer
Oral epithelial dysplasia
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2015 Collection
School of Dentistry Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
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Created: Mon, 02 Dec 2013, 22:20:46 EST by Roheen Gill on behalf of UQ Centre for Clinical Research