Experimentally induced blood-stage plasmodium vivax infection in healthy volunteers

McCarthy, James S., Griffin, Paul M., Sekuloski, Silvana, Bright, A. Taylor, Rockett, Rebecca, Looke, David, Elliott, Suzanne, Whiley, David, Sloots, Theo, Winzeler, Elizabeth A. and Trenholme, Katharine R. (2013) Experimentally induced blood-stage plasmodium vivax infection in healthy volunteers. Journal of Infectious Diseases, 208 10: 1688-1694. doi:10.1093/infdis/jit394

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Author McCarthy, James S.
Griffin, Paul M.
Sekuloski, Silvana
Bright, A. Taylor
Rockett, Rebecca
Looke, David
Elliott, Suzanne
Whiley, David
Sloots, Theo
Winzeler, Elizabeth A.
Trenholme, Katharine R.
Title Experimentally induced blood-stage plasmodium vivax infection in healthy volunteers
Formatted title
Experimentally induced blood-stage plasmodium vivax infection in healthy volunteers
Journal name Journal of Infectious Diseases   Check publisher's open access policy
ISSN 0022-1899
Publication date 2013-11-15
Year available 2013
Sub-type Article (original research)
DOI 10.1093/infdis/jit394
Volume 208
Issue 10
Start page 1688
End page 1694
Total pages 7
Place of publication Cary, NC, United States
Publisher Oxford University Press
Language eng
Formatted abstract
Background. Major impediments to development of vaccines and drugs for Plasmodium vivax malaria are the inability to culture this species and the extreme difficulty in undertaking clinical research by experimental infection.
Methods. A parasite bank was collected from a 49-year-old woman with P. vivax infection, characterized, and used in an experimental infection study.
Results. The donor made a full recovery from malaria after collection of a parasite bank, which tested negative for agents screened for in blood donations. DNA sequence analysis of the isolate indicated that it was clonal. Two subjects inoculated with the isolate became polymerase chain reaction positive on days 8 and 9, with onset of symptoms and positive blood smears on day 14, when they were treated with artemether-lumefantrine, with rapid clinical and parasitologic response. Transcripts of the parasite gene pvs25 that is expressed in gametocytes, the life cycle stage infectious to mosquitoes, were first detected on days 11 and 12.
Conclusions. This experimental system results in in vivo parasite growth, probably infectious to mosquitoes. It offers the opportunity to undertake studies previously impossible in P. vivax that will facilitate a better understanding of the pathology of vivax malaria and development of antimalarial drugs and vaccines.
Keyword Plasmodium vivax
Experimental infection
Sporozoite challenge model
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Queensland Children's Medical Research Institute Publications
School of Medicine Publications
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