Atractaspis aterrima toxins: The first insight into the molecular evolution of venom in side-stabbers

Terrat, Yves, Sunagar, Kartik, Fry, Bryan G., Jackson, Timothy N. W., Scheib, Holger, Fourmy, Rudy, Verdenaud, Maria, Blanchet, Guillaume, Antunes, Agostinho and Ducancel, Frederic (2013) Atractaspis aterrima toxins: The first insight into the molecular evolution of venom in side-stabbers. Toxins, 5 11: 1948-1964. doi:10.3390/toxins5111948

Author Terrat, Yves
Sunagar, Kartik
Fry, Bryan G.
Jackson, Timothy N. W.
Scheib, Holger
Fourmy, Rudy
Verdenaud, Maria
Blanchet, Guillaume
Antunes, Agostinho
Ducancel, Frederic
Title Atractaspis aterrima toxins: The first insight into the molecular evolution of venom in side-stabbers
Journal name Toxins   Check publisher's open access policy
ISSN 2072-6651
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.3390/toxins5111948
Open Access Status DOI
Volume 5
Issue 11
Start page 1948
End page 1964
Total pages 17
Place of publication Basel, Switzerland
Publisher M D P I AG
Language eng
Subject 3005 Toxicology
2307 Health, Toxicology and Mutagenesis
Abstract Although snake venoms have been the subject of intense research, primarily because of their tremendous potential as a bioresource for design and development of therapeutic compounds, some specific groups of snakes, such as the genus Atractaspis, have been completely neglected. To date only limited number of toxins, such as sarafotoxins have been well characterized from this lineage. In order to investigate the molecular diversity of venom from Atractaspis aterrima-the slender burrowing asp, we utilized a high-throughput transcriptomic approach completed with an original bioinformatics analysis pipeline. Surprisingly, we found that Sarafotoxins do not constitute the major ingredient of the transcriptomic cocktail; rather a large number of previously well-characterized snake venom-components were identified. Notably, we recovered a large diversity of three-finger toxins (3FTxs), which were found to have evolved under the significant influence of positive selection. From the normalized and non-normalized transcriptome libraries, we were able to evaluate the relative abundance of the different toxin groups, uncover rare transcripts, and gain new insight into the transcriptomic machinery. In addition to previously characterized toxin families, we were able to detect numerous highly-transcribed compounds that possess all the key features of venom-components and may constitute new classes of toxins.
Keyword Atractaspis
Venom gland
Venomous snake
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
Institute for Molecular Bioscience - Publications
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