Co-selection may explain high rates of ciprofloxacin non-susceptible Escherichia coli from retail poultry reared without prior fluoroquinolone exposure

Ingram, Paul Robert, Rogers, Benjamin A., Sidjabat, Hanna E., Gibson, Justine S. and Inglis, Timothy J.J. (2013) Co-selection may explain high rates of ciprofloxacin non-susceptible Escherichia coli from retail poultry reared without prior fluoroquinolone exposure. Journal of Medical Microbiology, 62 PART 11: 1743-1746. doi:10.1099/jmm.0.062729-0


Author Ingram, Paul Robert
Rogers, Benjamin A.
Sidjabat, Hanna E.
Gibson, Justine S.
Inglis, Timothy J.J.
Title Co-selection may explain high rates of ciprofloxacin non-susceptible Escherichia coli from retail poultry reared without prior fluoroquinolone exposure
Journal name Journal of Medical Microbiology   Check publisher's open access policy
ISSN 0022-2615
1473-5644
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1099/jmm.0.062729-0
Open Access Status Not yet assessed
Volume 62
Issue PART 11
Start page 1743
End page 1746
Total pages 4
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Subject 2726 Microbiology (medical)
2404 Microbiology
Abstract Australia has never permitted fluoroquinolone use in food-producing animals. We examined local retail poultry for contamination with fluoroquinolone non-susceptible Escherichia coli, then explored the hypothesis that their presence may be due to co-selection of resistance determinants. Between August and November 2010, samples from 30 locally produced, uncooked retail poultry carcasses from four different processing centres underwent selective enrichment culture for ciprofloxacin non-susceptible E. coli. Their chromosomal- and plasmid-mediated resistance determinants were characterized, and phylogenetic analysis and transformation experiments were performed. Unexpectedly, we found nine (30 %) of our small collection of poultry samples carried fluoroquinolone non-susceptible E. coli of which nearly half possessed aac(6′)-Ib-cr, a novel plasmid-mediated gene encoding an aminoglycoside acetylating enzyme that also confers fluoroquinolone resistance. All nine isolates were co-resistant to amoxicillin, gentamicin, tetracycline and trimethoprim/sulfamethoxazole - all antibiotic classes that are registered for use in poultry reared for food production within Australia. Their unique phylogenetic relatedness suggested clonal dissemination driven by non-fluoroquinolone selective pressures. aac(6′)-Ib-cr was successfully transformed and selected for using non-fluoroquinolone antibiotic pressure. Vertical and perhaps horizontal co-selection may be contributing to the emergence of fluoroquinolone resistance in poultry and could play a similar role in the human setting. This suggests that preservation of the usefulness of fluoroquinolones may require more than just restriction of their use in isolation from other interventions.
Keyword Microbiology
Microbiology
MICROBIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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