Co-selection may explain high rates of ciprofloxacin non-susceptible Escherichia coli from retail poultry reared without prior fluoroquinolone exposure

Ingram, Paul Robert, Rogers, Benjamin A., Sidjabat, Hanna E., Gibson, Justine S. and Inglis, Timothy J.J. (2013) Co-selection may explain high rates of ciprofloxacin non-susceptible Escherichia coli from retail poultry reared without prior fluoroquinolone exposure. Journal of Medical Microbiology, 62 PART 11: 1743-1746. doi:10.1099/jmm.0.062729-0


Author Ingram, Paul Robert
Rogers, Benjamin A.
Sidjabat, Hanna E.
Gibson, Justine S.
Inglis, Timothy J.J.
Title Co-selection may explain high rates of ciprofloxacin non-susceptible Escherichia coli from retail poultry reared without prior fluoroquinolone exposure
Journal name Journal of Medical Microbiology   Check publisher's open access policy
ISSN 0022-2615
1473-5644
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1099/jmm.0.062729-0
Volume 62
Issue PART 11
Start page 1743
End page 1746
Total pages 4
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Subject 2726 Microbiology (medical)
2404 Microbiology
Abstract Australia has never permitted fluoroquinolone use in food-producing animals. We examined local retail poultry for contamination with fluoroquinolone non-susceptible Escherichia coli, then explored the hypothesis that their presence may be due to co-selection of resistance determinants. Between August and November 2010, samples from 30 locally produced, uncooked retail poultry carcasses from four different processing centres underwent selective enrichment culture for ciprofloxacin non-susceptible E. coli. Their chromosomal- and plasmid-mediated resistance determinants were characterized, and phylogenetic analysis and transformation experiments were performed. Unexpectedly, we found nine (30 %) of our small collection of poultry samples carried fluoroquinolone non-susceptible E. coli of which nearly half possessed aac(6′)-Ib-cr, a novel plasmid-mediated gene encoding an aminoglycoside acetylating enzyme that also confers fluoroquinolone resistance. All nine isolates were co-resistant to amoxicillin, gentamicin, tetracycline and trimethoprim/sulfamethoxazole - all antibiotic classes that are registered for use in poultry reared for food production within Australia. Their unique phylogenetic relatedness suggested clonal dissemination driven by non-fluoroquinolone selective pressures. aac(6′)-Ib-cr was successfully transformed and selected for using non-fluoroquinolone antibiotic pressure. Vertical and perhaps horizontal co-selection may be contributing to the emergence of fluoroquinolone resistance in poultry and could play a similar role in the human setting. This suggests that preservation of the usefulness of fluoroquinolones may require more than just restriction of their use in isolation from other interventions.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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