Subcutaneous cholera toxin exposure induces potent CD103+ dermal dendritic cell activation and migration

Apte, Simon H., Redmond, Andrew M., Groves, Penny L., Schussek, Sophie, Pattinson, David J. and Doolan, Denise L. (2013) Subcutaneous cholera toxin exposure induces potent CD103+ dermal dendritic cell activation and migration. European Journal of Immunology, 43 10: 2707-2717. doi:10.1002/eji.201343475


Author Apte, Simon H.
Redmond, Andrew M.
Groves, Penny L.
Schussek, Sophie
Pattinson, David J.
Doolan, Denise L.
Title Subcutaneous cholera toxin exposure induces potent CD103+ dermal dendritic cell activation and migration
Journal name European Journal of Immunology   Check publisher's open access policy
ISSN 0014-2980
1521-4141
Publication date 2013-10-01
Year available 2013
Sub-type Article (original research)
DOI 10.1002/eji.201343475
Open Access Status
Volume 43
Issue 10
Start page 2707
End page 2717
Total pages 11
Place of publication Weinheim,Germany
Publisher Wiley
Language eng
Subject 2403 Immunology
2723 Immunology and Allergy
Formatted abstract
 CD103+ dermal dendritic cells (dDCs) are a recently described DC subset of the skin shown to be the principal migratory DCs capable of efficiently cross-presenting antigens and activating CD8+ T cells. Harnessing their activity would promote vaccine efficacy, but it has been unclear how this can be achieved. We tested a panel of adjuvants for their ability to affect dDCs. In comparison to the other adjuvants tested, the capacity of cholera toxin (CT) to induce the migration of dDCs was unique. Within 24 h of CT injection, large numbers of highly activated dDCs (including CD103+ dDCs) migrated to the draining lymph nodes and cross-presented coinjected antigens, potently activating naïve CD8+ T cells. Peptide vaccines adjuvanted with CT induced T-cell responses uniquely characterized by dynamic cytokine responses including the production of IL-2, and such vaccines were protective in situations reliant on CD8+ T-cell responses, including liver-stage Plasmodium challenge, or tumor challenge. This study is the first to examine the effects of adjuvants on CD103+ dDCs and identifies CT as a prototypical adjuvant for the activation of CD103+ dDCs, opening the way to development of vaccines and adjuvants that specifically target dDCs and generate effective CD8+ T-cell responses.
Keyword Adjuvants
CD8+ T cells
Cholera toxin
DCs
Vaccines
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
 
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