Early circulating biomarker detection using a wearable microprojection array skin patch

Coffey, Jacob W., Corrie, Simon R. and Kendall, Mark A. F. (2013) Early circulating biomarker detection using a wearable microprojection array skin patch. Biomaterials, 34 37: 9572-9583. doi:10.1016/j.biomaterials.2013.08.078

Author Coffey, Jacob W.
Corrie, Simon R.
Kendall, Mark A. F.
Title Early circulating biomarker detection using a wearable microprojection array skin patch
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2013.08.078
Open Access Status
Volume 34
Issue 37
Start page 9572
End page 9583
Total pages 12
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Subject 2502 Biomaterials
1502 Banking, Finance and Investment
2503 Ceramics and Composites
2211 Mechanics of Materials
1304 Biophysics
Abstract Microprojection array (MPA) skin patches selectively capture circulating biomarkers from the dermal layers of the skin, avoiding the need to extract, handle or process blood. Here we investigate the effect of improving biomarker capture invivo on MPA detection of a model biomarker (antigen-specific-IgG raised in response to Fluvax vaccine) in a murine model. First, we investigate targeting MPA penetration to biomarker rich regions of the skin by varying MPA penetration depth. We observed a 4-fold increase in biomarker capture from predominantly epidermal to deep dermal penetration (27±9μm-153±30μm penetration range). We then study the kinetics of biomarker capture by varying the contact time with skin from rapid application (less than 20min) to long term application (up to 24h) with a wearable MPA patch. We observed MPAs reproducibly captured detectable amounts of our model biomarker after 10minapplication and a greater than 6-fold increase in capture was observed up to 6happlication. Combining the effect of penetration depth and application time we obtained comparable early detection (after vaccination) of our model biomarker as a standard enzyme-linked immunosorbent assay (ELISA). We expect that integration of these devices with existing detection technologies has potential advantages in rapid diagnostic tests, particularly in cases where laboratory-based sample collection and processing is not available.
Keyword Biomarker
Microprojection array
Selective capture
Surface chemistry
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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