Immunological properties of extraembryonic human mesenchymal stromal cells derived from gestational tissue

Stubbendorff M., Deuse T., Hua X., Phan T.T., Bieback K., Atkinson K., Eiermann T.H., Velden J., Schroder C., Reichenspurner H., Robbins R.C., Volk H.-D. and Schrepfer S. (2013) Immunological properties of extraembryonic human mesenchymal stromal cells derived from gestational tissue. Stem Cells and Development, 22 19: 2619-2629. doi:10.1089/scd.2013.0043

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Author Stubbendorff M.
Deuse T.
Hua X.
Phan T.T.
Bieback K.
Atkinson K.
Eiermann T.H.
Velden J.
Schroder C.
Reichenspurner H.
Robbins R.C.
Volk H.-D.
Schrepfer S.
Title Immunological properties of extraembryonic human mesenchymal stromal cells derived from gestational tissue
Journal name Stem Cells and Development   Check publisher's open access policy
ISSN 1547-3287
Publication date 2013-10-01
Year available 2013
Sub-type Article (original research)
DOI 10.1089/scd.2013.0043
Open Access Status File (Author Post-print)
Volume 22
Issue 19
Start page 2619
End page 2629
Total pages 11
Place of publication New Rochelle, United States
Publisher Mary Ann Liebert
Language eng
Formatted abstract
Mesenchymal stromal cells (MSCs) have been isolated from many tissues, including gestational tissue. To date, a study comparing the properties and suitability of these cells in cell-based therapies is lacking. In this study, we compared the phenotype, proliferation rate, migration, immunogenicity, and immunomodulatory capabilities of human MSCs derived from umbilical cord lining (CL-MSCs), umbilical cord blood (CB-MSCs), placenta (P-MSCs), and Wharton's jelly (WJ-MSCs). Differences were noted in differentiation, proliferation, and migration, with CL-MSCs showing the highest proliferation and migration rates resulting in prolonged survival in immunodeficient mice. Moreover, CL-MSCs showed a prolongation in survival in xenogeneic BALB/c mice, which was attributed to their ability to dampen TH1 and TH2 responses. Weaker human cellular immune responses were detected against CL-MSCs and P-MSCs, which were correlated with their lower HLA I expression. Furthermore, HLA II was upregulated less substantially by CL-MSCs and CB-MSCs after IFN-γ stimulation. MSC types did not differ in indolamine 2,3-dioxygenase (IDO) expression after IFN-γ stimulation. Despite their lower IDO, HLA-G, and TGF-β1 expression, only CL-MSCs were able to reduce the release of IFN-γ by lymphocytes in a mixed lymphocyte reaction. In summary, CL-MSCs showed the best characteristics for cell-based strategies, as they are hypo-immunogenic and show high proliferation and migration rates. In addition, these studies show for the first time that although immunomodulatory molecules HLA-G, HLA-E, and TGF-β play an important role in MSC immune evasion, basal and induced HLA expression seems to be decisive in determining the immunogenicity of MSCs.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
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