Modulation of colonic inflammation in Mdr1a-/- mice by green tea polyphenols and their effects on the colon transcriptome and proteome

Barnett, Matthew P. G., Cooney, Janine M., Dommels, Yvonne E. M., Nones, Katia, Brewster, Diane T., Park, Zaneta, Butts, Christine A., McNabb, Warren C., Laing, William A. and Roy, Nicole C. (2013) Modulation of colonic inflammation in Mdr1a-/- mice by green tea polyphenols and their effects on the colon transcriptome and proteome. Journal of Nutritional Biochemistry, 24 10: 1678-1690. doi:10.1016/j.jnutbio.2013.02.007


Author Barnett, Matthew P. G.
Cooney, Janine M.
Dommels, Yvonne E. M.
Nones, Katia
Brewster, Diane T.
Park, Zaneta
Butts, Christine A.
McNabb, Warren C.
Laing, William A.
Roy, Nicole C.
Title Modulation of colonic inflammation in Mdr1a-/- mice by green tea polyphenols and their effects on the colon transcriptome and proteome
Formatted title
Modulation of colonic inflammation in Mdr1a-/- mice by green tea polyphenols and their effects on the colon transcriptome and proteome
Journal name Journal of Nutritional Biochemistry   Check publisher's open access policy
ISSN 0955-2863
1873-4847
Publication date 2013-10-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jnutbio.2013.02.007
Volume 24
Issue 10
Start page 1678
End page 1690
Total pages 13
Place of publication New York, United States
Publisher Elsevier
Language eng
Formatted abstract
Animal models are an important tool to understand the complex pathogenesis of inflammatory bowel diseases (IBDs). This study tested the anti-inflammatory potential of a green tea extract rich in polyphenols (GrTP) in the colon of the multidrug resistance targeted mutation ( Mdr1a-/-) mouse model of IBD. Insights into mechanisms responsible for this reduction in inflammation were gained using transcriptome and proteome analyses. Mice were randomly assigned to an AIN-76A (control) or GrTP-enriched diet. At 21 or 24 weeks of age, a colonic histological injury score was determined for each mouse, colon mRNA transcript levels were assessed using microarrays, and colon protein expression was measured using two-dimensional gel electrophoresis and liquid chromatography-mass spectrometry protein identification. Mean colonic histological injury score of GrTP-fed Mdr1a-/- mice was significantly lower compared to those fed the control diet. Microarray and proteomics analyses showed reduced abundance of transcripts and proteins associated with immune and inflammatory response and fibrinogenesis pathways, and increased abundance of those associated with xenobiotic metabolism pathways in response to GrTP, suggesting that its anti-inflammatory activity is mediated by multiple molecular pathways. Peroxisome proliferator-activated receptor-α and signal transducer and activator of transcription 1 appear to be two key molecules which regulate these effects. These results support the view that dietary intake of polyphenols derived from green tea can ameliorate intestinal inflammation in the colon of a mouse model of IBD, and are in agreement with studies suggesting that consumption of green tea may reduce IBD symptoms and therefore play a part in an overall IBD treatment regimen.
Keyword Epigallocatechin gallate (EGCG)
Gene expression
Inflammatory bowel diseases
Protein expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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