A cell-free approach to accelerate the study of protein-protein interactions in vitro

Sierecki, E., Giles, N., Polinkovsky, M., Moustaqil, M., Alexandrov, K. and Gambin, Y. (2013) A cell-free approach to accelerate the study of protein-protein interactions in vitro. Interface Focus, 3 5: . doi:10.1098/rsfs.2013.0018


Author Sierecki, E.
Giles, N.
Polinkovsky, M.
Moustaqil, M.
Alexandrov, K.
Gambin, Y.
Title A cell-free approach to accelerate the study of protein-protein interactions in vitro
Journal name Interface Focus   Check publisher's open access policy
ISSN 2042-8898
2042-8901
Publication date 2013-10-06
Year available 2013
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1098/rsfs.2013.0018
Volume 3
Issue 5
Total pages 11
Place of publication London, United Kingdom
Publisher The Royal Society Publishing
Language eng
Abstract Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.
Keyword Alphascreen
Drug discovery
In vitro protein expression
Protein- protein interactions
Single-molecule fluorescence
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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