The gray institute 'open' high-content, fluorescence lifetime microscopes

Barber, P. R., Tullis, I. D. C., Pierce, G. P., Newman, R. G., Prentice, J., Rowley, M. I., Matthews, D. R., Ameer-Beg, S. M. and Vojnovic, B. (2013) The gray institute 'open' high-content, fluorescence lifetime microscopes. Journal of Microscopy, 251 2: 154-167. doi:10.1111/jmi.12057

Author Barber, P. R.
Tullis, I. D. C.
Pierce, G. P.
Newman, R. G.
Prentice, J.
Rowley, M. I.
Matthews, D. R.
Ameer-Beg, S. M.
Vojnovic, B.
Title The gray institute 'open' high-content, fluorescence lifetime microscopes
Journal name Journal of Microscopy   Check publisher's open access policy
ISSN 0022-2720
Publication date 2013-01-01
Sub-type Article (original research)
DOI 10.1111/jmi.12057
Open Access Status DOI
Volume 251
Issue 2
Start page 154
End page 167
Total pages 14
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Abstract We describe a microscopy design methodology and details of microscopes built to this 'open' design approach. These demonstrate the first implementation of time-domain fluorescence microscopy in a flexible automated platform with the ability to ease the transition of this and other advanced microscopy techniques from development to use in routine biology applications. This approach allows easy expansion and modification of the platform capabilities, as it moves away from the use of a commercial, monolithic, microscope body to small, commercial off-the-shelf and custom made modular components. Drawings and diagrams of our microscopes have been made available under an open license for noncommercial use at Several automated high-content fluorescence microscope implementations have been constructed with this design framework and optimized for specific applications with multiwell plates and tissue microarrays. In particular, three platforms incorporate time-domain FLIM via time-correlated single photon counting in an automated fashion. We also present data from experiments performed on these platforms highlighting their automated wide-field and laser scanning capabilities designed for high-content microscopy. Devices using these designs also form radiation-beam 'end-stations' at Oxford and Surrey Universities, showing the versatility and extendibility of this approach.
Keyword FLIM
High-content microscopy
Tissue microarray
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Brain Institute Publications
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